Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


Thesis_Zahraa Al-Saedi.pdf (2.85 MB)

ETD Abstract Container

Abstract Header

Formulation and In vitro Evaluation of Cyclosporine A Inserts Prepared Using HPMC for Treating Dry Eye Disease

Al-Saedi, Zahraa Hasan Falhi
http://rave.ohiolink.edu/etdc/view?acc_num=mco1456095904

Abstract Details

2016, Master of Science (MS), University of Toledo, Pharmaceutical Sciences (Industrial Pharmacy).
Aim: The aim of this study was to develop and characterize a novel sustained-release drug delivery system of cyclosporine A (CsA) using hydroxypropyl methylcellulose (HPMC) and xanthan gum (XG) for treating dry eye disease (DED). Methods: Polymeric inserts of CsA were prepared using the solvent casting technique with a 23 full factorial design to evaluate the effect of HPMC, XG ratios and drug content on thickness, folding endurance, wettability and in vitro drug release. Inserts were also evaluated for drug content, moisture absorption and loss, and surface pH. Optimized CsA-loaded HPMC inserts were sterilized with UV light and evaluated for morphology, thermal analysis, FTIR, stability at 4°C, 25°C and 40°C, cytotoxicity in cultured bovine corneal endothelial cells, and anti-inflammatory effect in Jurkat T cells. Results: The addition of XG increased CsA release duration and enhanced the folding endurance of films. All films showed uniformity in drug content and thickness. Formulation F4 composed of 1% w/v HPMC and 0.25% w/v XG exhibited good folding endurance and sustained CsA release for up to 20h. Sterility testing of F4 using plate and direct inoculation confirmed the formulation sterility and validated the sterilization method. The formulation was found to be stable for at least 3 months at 4°C, 25°C and 40°C. No cytotoxicity was observed in cultured bovine corneal endothelial cells for up to 24 h. The anti-inflammatory effect of CsA was intact in ophthalmic inserts. Conclusion: In conclusion, the combination therapy with HPMC and CsA can be a potential once-a-day formulation for treating DED.
Sai Hanuman Sagar Boddu, Ph.D. (Advisor)
Pharmacy Sciences

Recommended Citations

Citations

  • Al-Saedi, Z. H. F. (2016). Formulation and In vitro Evaluation of Cyclosporine A Inserts Prepared Using HPMC for Treating Dry Eye Disease [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1456095904

    APA Style (7th edition)

  • Al-Saedi, Zahraa. Formulation and In vitro Evaluation of Cyclosporine A Inserts Prepared Using HPMC for Treating Dry Eye Disease. 2016. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=mco1456095904.

    MLA Style (8th edition)

  • Al-Saedi, Zahraa. "Formulation and In vitro Evaluation of Cyclosporine A Inserts Prepared Using HPMC for Treating Dry Eye Disease." Master's thesis, University of Toledo, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=mco1456095904

    Chicago Manual of Style (17th edition)

mco1456095904
2,154
© 2016, all rights reserved.
This open access ETD is published by University of Toledo Health Science Campus and OhioLINK.