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A Systematic Investigation of the Sulfation of Esculetin by the Human Cytosolic Sulfotransferases (SULTs)

Alatwi, Eid
http://rave.ohiolink.edu/etdc/view?acc_num=mco1493139209025322

Abstract Details

2017, Master of Science in Pharmaceutical Science (MSP), University of Toledo, Pharmaceutical Sciences (Pharmacology/Toxicology).
Esculetin is a coumarin derivative present in several plants that have been used as herbal medicine in China for many years. Esculetin has been shown to have a wide spectrum of biologic activities. However, the sulfation of esculetin has not yet been investigated. The present study was designed to identify and characterize the human cytosolic sulfotransferases (SULTs) that have the capacity to sulfate esculetin. A systematic investigation revealed that of the thirteen known human SULTs, SULT1A1, SULT1A3 and SULT1C4 showed the strongest sulfating activity toward esculetin. The pH-dependence and the kinetic parameters of these three SULTs in mediating the sulfation of esculetin were analyzed. HepG2 human hepatoma cells and Caco-2 human intestinal epithelial cells were shown to be able to sulfate esculetin under metabolic conditions. In addition, of the four human organ specimens (intestine, kidney, liver, and lung cytosols) examined, liver and intestine cytosols showed considerably higher esculetin-sulfating activity than lung and kidney cytosols. Taken together, these results provided valuable biochemical information for further investigation on the pharmacokinetics of esculetin in vivo.
Ming-Cheh Liu (Committee Chair)
Frederick E. Williams (Committee Member)
Zahoor Shah (Committee Member)
Biomedical Research; Pharmaceuticals; Pharmacology; Toxicology

Recommended Citations

Citations

  • Alatwi, E. (2017). A Systematic Investigation of the Sulfation of Esculetin by the Human Cytosolic Sulfotransferases (SULTs) [Master's thesis, University of Toledo]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=mco1493139209025322

    APA Style (7th edition)

  • Alatwi, Eid. A Systematic Investigation of the Sulfation of Esculetin by the Human Cytosolic Sulfotransferases (SULTs). 2017. University of Toledo, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=mco1493139209025322.

    MLA Style (8th edition)

  • Alatwi, Eid. "A Systematic Investigation of the Sulfation of Esculetin by the Human Cytosolic Sulfotransferases (SULTs)." Master's thesis, University of Toledo, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=mco1493139209025322

    Chicago Manual of Style (17th edition)

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© 2017, all rights reserved.
This open access ETD is published by University of Toledo Health Science Campus and OhioLINK.