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The Assessment and Application of Point Spread Function Deconvolution to High Pressure Fluorescence Microscopy Imaging

Haver, Thomas James
http://rave.ohiolink.edu/etdc/view?acc_num=miami1187612886

Abstract Details

2007, Master of Science, Miami University, Physics.
The effectiveness of image restoration techniques for use in high-pressure fluorescence microscopy are investigated and characterized. A quartz capillary, which acts as both a mechanical support and an optical window, is utilized as a high-pressure imaging chamber. We apply point-spread-function deconvolution using both a capillary and a flat slide control to analyze the relative contrast and resolution enhancement in fluorescent images. The asymmetry in the lateral and axial directions is examined for all point spread functions. Spatial deconvolution is shown to be an effective method at high pressures to reduce out-of-focus fluorescence in microscope images by reversing the effects of optical aberrations. The results confirm there is significant image improvement using either low- or high-numerical aperture objectives. Lateral resolution is improved over raw images by applying calculated and measured point spread functions, with both processes yielding comparable results.
Paul Urayama (Advisor)
154 p.
High Pressure; Microscopy; Deconvolution; PSF; Imaging; Resolution; Contrast

Recommended Citations

Citations

  • Haver, T. J. (2007). The Assessment and Application of Point Spread Function Deconvolution to High Pressure Fluorescence Microscopy Imaging [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1187612886

    APA Style (7th edition)

  • Haver, Thomas. The Assessment and Application of Point Spread Function Deconvolution to High Pressure Fluorescence Microscopy Imaging. 2007. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1187612886.

    MLA Style (8th edition)

  • Haver, Thomas. "The Assessment and Application of Point Spread Function Deconvolution to High Pressure Fluorescence Microscopy Imaging." Master's thesis, Miami University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=miami1187612886

    Chicago Manual of Style (17th edition)

miami1187612886
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© 2007, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.