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ALKYLAMMONIUM FORMATE IONIC LIQUIDS AS SOLVENTS FOR FLUORESCENCE AND LIQUID CHROMATOGRAPHY METHODS

Dotlich, Erin Michele
http://rave.ohiolink.edu/etdc/view?acc_num=miami1208983119

Abstract Details

2008, Master of Science, Miami University, Chemistry and Biochemistry.
Three alkylammonium formate ionic liquids (ILs) [ethylammonium formate (EAF), propylammonium formate (PAF) and butylammonium formate (BAF)] have been studied as alternative solvents with fluorescence detection. The fluorescence spectra of naproxen, riboflavin and epinephrine in water and these ILs have been compared. Although there is decreased fluorescence intensity in the ILs, there is no wavelength shift and fluorescence spectra are still resolved. Using a monolithic column, naproxen and tryptophan are separated using a MeOH-water or EAF-water mobile phase. The mobile phase containing EAF shows improved peak symmetry and efficiency, although solvent strength for MeOH is 3.3, but is only 2.2 for EAF. Riboflavin and 9-methyacridine as well as a combination of indoles were separated using EAF as a mobile phase modifier. Finally, these ILs were studied as enzyme diluents for cytochrome C and bovine serum albumin. Results showed that these ILs are less denaturing to these enzymes than common organic solvents.
Neil Danielson, PhD (Advisor)
Thomas Riechel, PhD (Committee Chair)
Benjamin Gung, PhD (Committee Member)
Andre Sommer, PhD (Committee Member)
120 p.
Chemistry
Ionic Liquids HPLC High Performance Liquid Chromatography Fluorescence detection Alkylammonium Formate ethylammonium butylammonium propylammonium

Recommended Citations

Citations

  • Dotlich, E. M. (2008). ALKYLAMMONIUM FORMATE IONIC LIQUIDS AS SOLVENTS FOR FLUORESCENCE AND LIQUID CHROMATOGRAPHY METHODS [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1208983119

    APA Style (7th edition)

  • Dotlich, Erin. ALKYLAMMONIUM FORMATE IONIC LIQUIDS AS SOLVENTS FOR FLUORESCENCE AND LIQUID CHROMATOGRAPHY METHODS. 2008. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1208983119.

    MLA Style (8th edition)

  • Dotlich, Erin. "ALKYLAMMONIUM FORMATE IONIC LIQUIDS AS SOLVENTS FOR FLUORESCENCE AND LIQUID CHROMATOGRAPHY METHODS." Master's thesis, Miami University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=miami1208983119

    Chicago Manual of Style (17th edition)

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This open access ETD is published by Miami University and OhioLINK.