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miami1343970397.pdf (15.94 MB)
ETD Abstract Container
Abstract Header
A Hydrostatic Pressure Perfusion System for Biological Systems
Author Info
Huff, Alison
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=miami1343970397
Abstract Details
Year and Degree
2012, Master of Science, Miami University, Physics.
Abstract
A hydrostatic pressure perfusion system with two closed fluid reservoirs has been constructed and characterized. The system utilizes two stainless steel pressure generators connected to gearheads. Stepper motors are connected to the gearheads and are used to provide constant fluid flow throughout the system. We communicate with both motors through a hub, such that we can monitor the internal pressure of the system with a pressure gauge. Fluorescence has been used to detect changes in emission spectra between the different fluids in the reservoirs. We have also demonstrated that we can trap Saccharomyces cerevisiae, or baker's yeast, in a quartz capillary tube and detect spectral changes while monitoring the internal pressure of the system.
Committee
Paul Urayama, PhD (Advisor)
Michael Pechan, PhD (Committee Member)
Herbert Jaeger, PhD (Committee Member)
Subject Headings
Biophysics
;
Physics
Keywords
hydrostatic pressure
;
perfusion system
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Citations
Huff, A. (2012).
A Hydrostatic Pressure Perfusion System for Biological Systems
[Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1343970397
APA Style (7th edition)
Huff, Alison.
A Hydrostatic Pressure Perfusion System for Biological Systems.
2012. Miami University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=miami1343970397.
MLA Style (8th edition)
Huff, Alison. "A Hydrostatic Pressure Perfusion System for Biological Systems." Master's thesis, Miami University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=miami1343970397
Chicago Manual of Style (17th edition)
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Document number:
miami1343970397
Download Count:
220
Copyright Info
© 2012, some rights reserved.
A Hydrostatic Pressure Perfusion System for Biological Systems by Alison Huff is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Miami University and OhioLINK.