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BIOPHYSICAL CHARACTERIZATION OF ASF/SF2’S INTERACTION WITH SPLICE SITE A7 IN THE HIV GENOME

Kochert, Brent Andrew
http://rave.ohiolink.edu/etdc/view?acc_num=miami1354600074

Abstract Details

2012, Master of Science, Miami University, Chemistry and Biochemistry.
The HIV genome is synthesized as a 9.5kb polycistronic transcript that undergoes alternative splicing to produce the complete viral protein compliment. Within the HIV genome both donor and acceptor sites are coupled together to determine which of the over 40 transcripts are made and thus which viral proteins are produced. Host proteins are responsible for both the up regulation and down regulation of these sites and play a key role in viral protein production. ASF, is a serine arginine rich protein that is capable of binding splice site A7 and upregulating splicing. The RNA recognition motif domains (RRMs) were studied both separately and together with the complete splice site as well as exonic splicing enhancer 3 (ESE3). A major goal of this study was to identify which domain has a greater responsibility in binding the splice site. In order to understand this, a series of electrophoretic mobility shift assays were run. It is seen that RRM-1 has a higher affinity for both the complete splice site as well as ESE3.
Christopher Makaroff, PhD (Committee Chair)
Carole Dabney-Smith, PhD (Committee Member)
Natosha Finley, PhD (Committee Member)
David Tierney, PhD (Committee Member)
103 p.
Biochemistry; Biophysics; Chemistry
ASF; SF2; Alternative Splicing Factor; Splicing Factor 2; Exonic Splicing Enhancer 3; ESE3; Alternative Splicing; Splice Site A7; Biophysical; HIV Genome

Recommended Citations

Citations

  • Kochert, B. A. (2012). BIOPHYSICAL CHARACTERIZATION OF ASF/SF2’S INTERACTION WITH SPLICE SITE A7 IN THE HIV GENOME [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1354600074

    APA Style (7th edition)

  • Kochert, Brent. BIOPHYSICAL CHARACTERIZATION OF ASF/SF2’S INTERACTION WITH SPLICE SITE A7 IN THE HIV GENOME. 2012. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1354600074.

    MLA Style (8th edition)

  • Kochert, Brent. "BIOPHYSICAL CHARACTERIZATION OF ASF/SF2’S INTERACTION WITH SPLICE SITE A7 IN THE HIV GENOME." Master's thesis, Miami University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=miami1354600074

    Chicago Manual of Style (17th edition)

miami1354600074
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© 2012, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.