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Towards a System for Nanosecond-Gated, Fluorescence Based Monitoring of Cellular Responses to High Hydrostatic Pressures

Long, Zachary C
http://rave.ohiolink.edu/etdc/view?acc_num=miami1375882251

Abstract Details

2013, Master of Science, Miami University, Physics.
A time-gated fluorescence spectroscopy system capable of nanosecond gating and picosecond control of gate delays is presented. Used in conjunction with pulsed excitation, the system is capable of tracking the temporal evolution of the fluorescence spectrum from solution samples. The system uses a nitrogen discharge laser as the excitation source and a time-gated intensified CCD detector coupled to a spectrograph. Precise synchronization between the laser pulse and ICCD gate is achieved using a constant-fraction optical discriminator. In addition a high-pressure perfusion system is constructed with an optically compatible high pressure chamber for use in conjunction with fluorescence spectroscopy. System characterizations for both systems are presented, for example, the ability to both spectrally and temporally resolve the content of fluorophore mixtures is confirmed. Time resolved and high pressure perfusion data is collected using Saccharomyces cerevisiae. Biotechnological applications are highlighted, including gated spectroscopy for the real-time monitoring of metabolic activity via measurement of endogenous cellular fluorescence.
Paul Urayama (Advisor)
Herbert Jaegar (Committee Member)
Stephen Alexander (Committee Member)
83 p.
Biophysics; Physics
Biophysics; Cellular Metabolism; LIF; Spectroscopy; Biological Monitoring; Pressure, Time-Resolved; Perfusion

Recommended Citations

Citations

  • Long, Z. C. (2013). Towards a System for Nanosecond-Gated, Fluorescence Based Monitoring of Cellular Responses to High Hydrostatic Pressures [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1375882251

    APA Style (7th edition)

  • Long, Zachary. Towards a System for Nanosecond-Gated, Fluorescence Based Monitoring of Cellular Responses to High Hydrostatic Pressures. 2013. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1375882251.

    MLA Style (8th edition)

  • Long, Zachary. "Towards a System for Nanosecond-Gated, Fluorescence Based Monitoring of Cellular Responses to High Hydrostatic Pressures." Master's thesis, Miami University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=miami1375882251

    Chicago Manual of Style (17th edition)

miami1375882251
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© 2013, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.