Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


Stempinski_thesis.pdf (4.06 MB)

ETD Abstract Container

Abstract Header

SYN3 in Chloroplasts of Arabidopsis thaliana: Effects of Knockdown and Overexpression and Localization Techniques

Stempinski, Erin S
http://rave.ohiolink.edu/etdc/view?acc_num=miami1376057385

Abstract Details

2013, Master of Science, Miami University, Botany.
Cohesins are complexes of proteins involved in sister chromatid cohesion during mitosis and meiosis as well as DNA repair and transcriptional regulation. One subunit of this protein complex, called SYN3, has been found in chloroplasts of Arabidopsis thaliana. In an effort to determine the role of SYN3 in chloroplasts, we examined RNAi knockdown and overexpression lines. Our results indicate that knockdown and overexpression of SYN3 do not affect chloroplast size or number, but knockdown does affect senescence, with earlier senescence, disruption of chloroplast structure, and fewer autophagosomes. Localization shows SYN3 distributed throughout the chloroplast stroma, but no co-localization with DNA. Several methods were used to localize SYN3 for TEM. High paraformaldehyde and low glutaraldehyde concentrations in sodium phosphate buffer and embedment in LR White preserved ultrastructural integrity and retained antigenicity. Post-section staining with osmium tetroxide vapors allowed for visualization of the thylakoids within the chloroplast without introducing artifacts.
Richard Edelmann, PhD (Advisor)
65 p.
Botany
SYN3; Arabidopsis thaliana; chloroplasts; transmission electron microscopy; TEM; immunolocalization; fixation

Recommended Citations

Citations

  • Stempinski, E. S. (2013). SYN3 in Chloroplasts of Arabidopsis thaliana: Effects of Knockdown and Overexpression and Localization Techniques [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1376057385

    APA Style (7th edition)

  • Stempinski, Erin. SYN3 in Chloroplasts of Arabidopsis thaliana: Effects of Knockdown and Overexpression and Localization Techniques. 2013. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1376057385.

    MLA Style (8th edition)

  • Stempinski, Erin. "SYN3 in Chloroplasts of Arabidopsis thaliana: Effects of Knockdown and Overexpression and Localization Techniques." Master's thesis, Miami University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=miami1376057385

    Chicago Manual of Style (17th edition)

miami1376057385
543
© 2013, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.
Release 3.2.12