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LappinThesis .Final.pdf (1.39 MB)
ETD Abstract Container
Abstract Header
REDEFINITION OF THE PSEUDOAUTOSOMAL BOUNDARY OF THE CARICA PAPAYA SEX CHROMOSOMES.
Author Info
Lappin, Fiona M
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=miami1376205368
Abstract Details
Year and Degree
2013, Master of Science, Miami University, Botany.
Abstract
The sex chromosomes of Carica papaya are characterized by a small region of suppressed recombination flanked by two larger, recombining pseudoautosomal regions (PARs). To better understand the evolution of the sex chromosomes, I analyzed polymorphism across the entire X chromosome using next generation resequencing. In addition, I used traditional sequencing to analyze polymorphism at nine loci close to the PAR boundary. Two of these nine loci exhibit clear molecular signatures of X/Y-linkage. The other seven loci have autosomal patterns of polymorphism. By mapping these along the X chromosome, I was able to identify a ~95kb region where the boundary between the PAR and X lies, reducing the previous estimated size of the X by ~2Mbp. A clearly defined PAR border will allow for further investigation into the evolution of expansion of the PAR, especially when studied in a subject that has relatively young sex chromosomes like that of papaya.
Committee
Richard Moore (Advisor)
Micheal Vincent (Committee Member)
Chun Liang (Committee Member)
Bruce Cochrane (Committee Member)
Pages
63 p.
Subject Headings
Biology
;
Botany
;
Genetics
Keywords
Carica papaya
;
pseudoautosomal regions
;
sex chromosomes
;
X chromosome
;
PAR border
;
patterns of polymorphism
;
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Citations
Lappin, F. M. (2013).
REDEFINITION OF THE PSEUDOAUTOSOMAL BOUNDARY OF THE CARICA PAPAYA SEX CHROMOSOMES.
[Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1376205368
APA Style (7th edition)
Lappin, Fiona.
REDEFINITION OF THE PSEUDOAUTOSOMAL BOUNDARY OF THE CARICA PAPAYA SEX CHROMOSOMES.
2013. Miami University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=miami1376205368.
MLA Style (8th edition)
Lappin, Fiona. "REDEFINITION OF THE PSEUDOAUTOSOMAL BOUNDARY OF THE CARICA PAPAYA SEX CHROMOSOMES." Master's thesis, Miami University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=miami1376205368
Chicago Manual of Style (17th edition)
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Document number:
miami1376205368
Download Count:
342
Copyright Info
© 2013, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.