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2014-08-07_Hall_Thesis.pdf (1.37 MB)

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KERATIN HYDROGELS FOR ANTIBIOTIC DELIVERY IN WOUND HEALING APPLICATIONS: DEVELOPMENT OF HPLC METHODS TO CHARACTERIZE RELEASE

Hall, Rachael Catherine
http://rave.ohiolink.edu/etdc/view?acc_num=miami1407435625

Abstract Details

2014, Master of Science, Miami University, Chemical, Paper and Biomedical Engineering.
In the United States, 450,000 burn injuries receive medical treatment per year1 with approximately 2% experiencing wound infection2. Current clinical paradigms such as Epicel, Apligraf, and Alloderm may provide wound coverage and promote wound healing, but do not incorporate antibiotics to fight microbial infection. Thus, dressings or materials that can promote healing while preventing infection are an important unmet clinical need. One possible solution is a biomaterial loaded with antibiotics. Off-the-shelf use of hydrogels made from keratin, collagen, and fibronectin proteins are attractive because of their physiochemical capacity to facilitate skin healing by promoting appropriate cellular responses. This thesis focuses on keratin hydrogels due to their ability to resist proteolytic degradation, promote vascularization, and support cellular ingrowth. The principal hypothesis of these experiments is that antibiotic loaded keratin hydrogels promote sustained release in a manner correlated with their degradation. To investigate this hypothesis, this thesis developed high performance liquid chromatography (HPLC) methods for detection of antibiotics in the presence of keratin proteins. Specifically, the results of this study helped to quantify antibiotic release from keratin hydrogels, and to characterize previous results indicating the sustained release of three specific antibiotics, cephazolin, ciprofloxacin, and neomycin. The results of these studies demonstrate the suitability of keratin hydrogels for antibiotic release and support conducting pre-clinical animal testing as well as possible FDA submissions for future human trials.
Justin Saul, PhD (Advisor)
Jason Berberich, PhD (Committee Member)
Neil Danielson, PhD (Committee Member)
73 p.
Engineering
KERATIN HYDROGELS, ANTIBIOTIC DELIVERY IN WOUND HEALING APPLICATIONS, DEVELOPMENT OF HPLC METHODS TO CHARACTERIZE RELEASE

Recommended Citations

Citations

  • Hall, R. C. (2014). KERATIN HYDROGELS FOR ANTIBIOTIC DELIVERY IN WOUND HEALING APPLICATIONS: DEVELOPMENT OF HPLC METHODS TO CHARACTERIZE RELEASE [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1407435625

    APA Style (7th edition)

  • Hall, Rachael. KERATIN HYDROGELS FOR ANTIBIOTIC DELIVERY IN WOUND HEALING APPLICATIONS: DEVELOPMENT OF HPLC METHODS TO CHARACTERIZE RELEASE. 2014. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1407435625.

    MLA Style (8th edition)

  • Hall, Rachael. "KERATIN HYDROGELS FOR ANTIBIOTIC DELIVERY IN WOUND HEALING APPLICATIONS: DEVELOPMENT OF HPLC METHODS TO CHARACTERIZE RELEASE." Master's thesis, Miami University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=miami1407435625

    Chicago Manual of Style (17th edition)

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© 2014, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.