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Use of metabolomic studies to understand the chemical role of ETHE1 in Arabidopsis thaliana

Wipulaguna, M.A. Anushika Shiromi
http://rave.ohiolink.edu/etdc/view?acc_num=miami1417604333

Abstract Details

2014, Master of Science, Miami University, Chemistry and Biochemistry.
Mutations in the human ethylmalonic encephalophathy (ETHE1) protein result a chronic metabolic disease and infants born with this disease will live only the first decade of their lives. Common symptoms of this disease are chronic diarrhea, a delay in neural development, and lactic acidemia. ETHE 1 is found in a wide range of organisms including bacteria, plants and animals. Mutations in plant ETHE1 results in alterations in endosperm development and a delay in embryo development, but the biochemical role of ETHE1 in plants is unclear. In this study we analyzed the metabolite levels in two Arabidopsis suspension cell lines containing different ETHE1 proteins. Metabolites were screened using NMR and principal component analysis was performed to identify metabolites that exhibited significant concentration differences. In this study we found that all the cells were undergoing stress and metabolites associated with a response to stress were elevated. It was also observed that the ETHE1 overexpression cell lines exhibited stress tolerance.
Christopher Makaroff, PhD (Advisor)
Ann Hagerman, PhD (Committee Chair)
Michael Kennedy, PhD (Committee Member)
Carole Dabney-Smith, PhD (Committee Member)
95 p.
Biochemistry; Chemistry
Metabolomics, Arabidopsis thaliana

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Citations

  • Wipulaguna, M.A. A. S. (2014). Use of metabolomic studies to understand the chemical role of ETHE1 in Arabidopsis thaliana [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1417604333

    APA Style (7th edition)

  • Wipulaguna, M.A. Anushika. Use of metabolomic studies to understand the chemical role of ETHE1 in Arabidopsis thaliana. 2014. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1417604333.

    MLA Style (8th edition)

  • Wipulaguna, M.A. Anushika. "Use of metabolomic studies to understand the chemical role of ETHE1 in Arabidopsis thaliana." Master's thesis, Miami University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=miami1417604333

    Chicago Manual of Style (17th edition)

miami1417604333
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© 2014, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.