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Master thesis_Yi-Ting Lai.pdf (10.06 MB)

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Enhancer identification and activity evaluation in the red flour beetle, Tribolium castaneum

Lai, Yi-Ting
http://orcid.org/0000-0001-8631-3208
http://rave.ohiolink.edu/etdc/view?acc_num=miami1484050187954831

Abstract Details

2017, Master of Science, Miami University, Biology.
Changes in the gene regulatory networks (GRNs) are the major driving force of morphological diversity. Evolution of cis-properties (non-coding DNA elements such as enhancers) often plays important roles in the production of diverse morphology, as changes in cis-properties tend to be less deleterious. However, studies on enhancers in species outside of established model systems are often limited because of the lacking a well-established enhancer identification and evaluation strategy. In this study, I used FAIRE (Formaldehyde Assisted Identification of Regulatory Elements) to identify enhancers in the red flour beetle, Tribolium castaneum. Furthermore, by using a construct with the Drosophila synthetic core promoter (DSCP), I established a functional reporter assay system in Tribolium. This reporter assay system, in combination with FAIRE-based chromatin profiling, will make the research on enhancers more accessible, which will provide us with more insights into the evolution of the regulatory mechanisms underlying morphological diversity.
Yoshinori Tomoyasu (Advisor)
Paul James (Committee Member)
Joyce Fernandes (Committee Member)
32 p.
Biology; Developmental Biology; Evolution and Development; Genetics; Molecular Biology
Enhancer; reporter assay; Tribolium; chromatin; FAIRE-seq

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Citations

  • Lai, Y.-T. (2017). Enhancer identification and activity evaluation in the red flour beetle, Tribolium castaneum [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1484050187954831

    APA Style (7th edition)

  • Lai, Yi-Ting. Enhancer identification and activity evaluation in the red flour beetle, Tribolium castaneum. 2017. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1484050187954831.

    MLA Style (8th edition)

  • Lai, Yi-Ting. "Enhancer identification and activity evaluation in the red flour beetle, Tribolium castaneum." Master's thesis, Miami University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=miami1484050187954831

    Chicago Manual of Style (17th edition)

miami1484050187954831
367
© 2017, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.