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Aman MS Thesis (07-21-2017).pdf (2.01 MB)

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Insights into the Chloroplast Tat Mechanism of Transport

Habtemichael, Aman Gebreyohannes
http://orcid.org/0000-0002-0473-1068
http://rave.ohiolink.edu/etdc/view?acc_num=miami1500654052065743

Abstract Details

2017, Master of Science, Miami University, Chemistry and Biochemistry.
The chloroplast Twin arginine transport (cpTat) system transports folded proteins across the thylakoids in chloroplast using proton motive force as the only source of energy. The cpTat is composed of three components; Tha4, Hcf106, and cpTatC. Hcf106 and cpTatC form a receptor complex where the precursor binds. Tha4 is found as separate homo-oligomers that assemble the receptor-bound substrate to complete the translocation. Though the overall model for the transport cycle is established, detailed sequential events remain to be elucidated. This thesis mainly probes conformational change of Hcf106 upon substrate binding using cysteine accessibility technique and interaction points between cpTatC first stromal domain (S1) and precursor mature domain using disulfide crosslinking to understand their direct contribution to the actual translocation event. We detected a more buried Hcf106 amphipathic helix (APH) after precursor binds. Besides, interactions were detected between cpTatC S1 and precursor mature domain. Altogether, our data supports a model that depicts Hcf106 APH membrane weakening and cpTatC insertase activity to promote the translocation of the precursor.
Carole Dabney-Smith, Ph.D. (Advisor)
Rick Page, Ph.D. (Committee Chair)
Jiangjiang Zhu, Ph.D. (Committee Member)
Heeyoung Tai , Ph.D. (Committee Member)
75 p.
Biochemistry
Thylakoid; protein transport; chloroplast twin arginine transport;

Recommended Citations

Citations

  • Habtemichael, A. G. (2017). Insights into the Chloroplast Tat Mechanism of Transport [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1500654052065743

    APA Style (7th edition)

  • Habtemichael, Aman. Insights into the Chloroplast Tat Mechanism of Transport. 2017. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1500654052065743.

    MLA Style (8th edition)

  • Habtemichael, Aman. "Insights into the Chloroplast Tat Mechanism of Transport." Master's thesis, Miami University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=miami1500654052065743

    Chicago Manual of Style (17th edition)

miami1500654052065743
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This open access ETD is published by Miami University and OhioLINK.