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Understanding the Regulatory Mechanism of BfmR in Acinetobacter baumannii ATCC 19606T

Mack, Lydia Eileen
http://orcid.org/0000-0003-4154-9297
http://rave.ohiolink.edu/etdc/view?acc_num=miami1561671121598468

Abstract Details

2019, Master of Science, Miami University, Microbiology.
The BfmRS two-component regulatory system of Acinetobacter baumannii plays roles in biofilm formation, pili production, capsule production, and other functions essential for colonization and persistence. Based on previously-collected RNA sequencing data comparing wild-type A. baumannii ATCC 19606T to an isogenic BfmR-deficient strain, we hypothesized that BfmR negatively regulates transcription of the genes for acinetobactin biosynthesis, secretion, and uptake for A. baumannii ATCC 19606T. Considering the recent identification of a BfmR-binding inverted repeat sequence, we also hypothesized that BfmR directly regulates the transcription of genes containing the BfmR-binding site upstream of the predicted start codon. Experimental analyses performed in this work included immunoblotting assays, qRT-PCR, electrophoretic mobility shift assays, molecular cloning techniques, and biofilm formation assays. The data acquired in this thesis demonstrate that BfmR is capable of repressing the expression of acinetobactin biosynthesis, secretion, and uptake genes. We also present information regarding the ability of BfmR to exhibit direct and indirect transcriptional regulation of metabolite transport and pili production genes.
Luis Actis (Advisor)
Mitchell Balish (Committee Member)
Timothy Wilson (Committee Member)
Michael Crowder (Committee Member)
86 p.
Microbiology
Acinetobacter; baumannii; two component system; BfmR; regulation; microbiology

Recommended Citations

Citations

  • Mack, L. E. (2019). Understanding the Regulatory Mechanism of BfmR in Acinetobacter baumannii ATCC 19606T [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1561671121598468

    APA Style (7th edition)

  • Mack, Lydia. Understanding the Regulatory Mechanism of BfmR in Acinetobacter baumannii ATCC 19606T. 2019. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1561671121598468.

    MLA Style (8th edition)

  • Mack, Lydia. "Understanding the Regulatory Mechanism of BfmR in Acinetobacter baumannii ATCC 19606T." Master's thesis, Miami University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=miami1561671121598468

    Chicago Manual of Style (17th edition)

miami1561671121598468
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© 2019, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.