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Interaction between Polyphenols and Metalloproteins

Fu, Meiling
http://orcid.org/0000-0003-3699-9891
http://rave.ohiolink.edu/etdc/view?acc_num=miami1650295570298551

Abstract Details

2022, Master of Science, Miami University, Cell, Molecular and Structural Biology (CMSB).
Polyphenols have been investigated for their roles in plant science, including resistance to pathogens and predators and potential human health benefits. Polyphenols are abundant in environment and are structurally diverse. My project focused on polyphenols and two metal-protein systems. One project explored epigallocatechin gallate (EGCg) and the serum albumin-Cu(II) complex. EGCg is a bioactive phytochemical with antioxidant activity, while copper is an essential micronutrient. Copper binds to serum albumin at two sites, the high affinity NTS site and the lower affinity MBS site. EGCg also binds to albumin and chelates and possibly reduces Cu(II). I evaluated whether Cu(II), EGCg and albumin form a ternary complex using fluorescence, UV/Vis and EPR spectroscopy. Our results suggest EGCg reduces serum albumin-Cu(II)MBS to Cu(I) but has minimum effects on albumin-Cu(II)NTS. Another project focused on the possible degradation pathway of condensed tannin by the soil manganese peroxidase (MnP) by using monomers, (+)-catechin, (-)-epicatechin, and dimer B2. Condensed tannin degradation by the soil microbiome, which is critical to carbon cycle and ecology, is vastly unknown. Our findings highlight the importance of enzyme catalyzed reaction in the oxidation related CT degradation, and we also propose the possible role of structural features in the oxidation of CT.
Ann Hagerman (Advisor)
Meixia Zhao (Committee Member)
Carole Dabney-Smith (Committee Member)
85 p.
Biochemistry; Pharmacology; Soil Sciences
Polyphenols, tannins, epigallocatechin gallate, serum albumin, copper, manganese peroxidase

Recommended Citations

Citations

  • Fu, M. (2022). Interaction between Polyphenols and Metalloproteins [Master's thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=miami1650295570298551

    APA Style (7th edition)

  • Fu, Meiling. Interaction between Polyphenols and Metalloproteins. 2022. Miami University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=miami1650295570298551.

    MLA Style (8th edition)

  • Fu, Meiling. "Interaction between Polyphenols and Metalloproteins." Master's thesis, Miami University, 2022. http://rave.ohiolink.edu/etdc/view?acc_num=miami1650295570298551

    Chicago Manual of Style (17th edition)

miami1650295570298551
139
© 2022, all rights reserved.
This open access ETD is published by Miami University and OhioLINK.