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The Role of Orphanin FQ (OFQ/N) in Mediating Adaptation to Chronic Stress

Kelbley, Jennifer E.
http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1146244076

Abstract Details

2006, Bachelor of Science, Miami University, College of Arts and Sciences - Zoology.
Stress causes complex neuroendocrine changes that include activation of the hypothalamic-pituitary-adrenal (HPA) axis, ultimately increasing levels of corticosterone (CORT) in the bloodstream. The purpose of this study was to determine the role of the opiate Orphanin FQ/Nociceptin (OFQ/N) in mediating adaptation to chronic stress. To do this, CORT levels were measured under resting and stress conditions in OFQ/N knockout mice. There was no significant difference in the basal CORT levels of knockout mice compared to their wild type littermates. An intermittent shaker stress was used to elicit the stress response in these mice. The shaker was automatically controlled to deliver 5 minute bouts of shaking every half hour. Knockout and wild type mice were exposed to 1, 3, 6 or 30 of these bouts of stress. All treatment groups were statistically higher than the controls which received no stress (p<0.01). The 3 bout group was the only treatment group in which knockout CORT levels were statistically higher than wild type levels (p=0.03). The CORT levels for both genotypes began to decline in the 6 bout and 30 bout groups. These results suggest that the HPA axis in OFQ/N knockout mice is more sensitive to stress, but adaptation proceeds normally. Western blot analysis was used to quantify expression levels of the ì-opioid receptor to determine if other endogenous opiates were compensating for the lack of OFQ/N in knockout mice. The western blot results were extremely variable in all treatment groups, and no conclusion could be made.
Phyllis Callahan (Advisor)
37 p.
stress; OFQ/N; mu opioid receptor; HPA axis; corticosterone

Recommended Citations

Citations

  • Kelbley, J. E. (2006). The Role of Orphanin FQ (OFQ/N) in Mediating Adaptation to Chronic Stress [Undergraduate thesis, Miami University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1146244076

    APA Style (7th edition)

  • Kelbley, Jennifer. The Role of Orphanin FQ (OFQ/N) in Mediating Adaptation to Chronic Stress. 2006. Miami University, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1146244076.

    MLA Style (8th edition)

  • Kelbley, Jennifer. "The Role of Orphanin FQ (OFQ/N) in Mediating Adaptation to Chronic Stress." Undergraduate thesis, Miami University, 2006. http://rave.ohiolink.edu/etdc/view?acc_num=muhonors1146244076

    Chicago Manual of Style (17th edition)

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© 2006, all rights reserved.
This open access ETD is published by Miami University Honors Theses and OhioLINK.
Release 3.2.12