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Masters Final 5-28-14.pdf (2.62 MB)

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Differential Loss of Bidirectional Axonal Transport with Structural Persistence Within The Same Optic Projection of the DBA/2J Glaucomatous Mouse

Smith, Matthew Alan
http://rave.ohiolink.edu/etdc/view?acc_num=ne2mh1401723180

Abstract Details

2014, Master of Science, Northeast Ohio Medical University, Integrated Pharmaceutical Medicine.
Glaucoma, the second leading cause of blindness worldwide, involves the degeneration of retinal ganglion cell bodies and their axons resulting in progressive vision loss. Much like other neurodegenerations, deficits in axonal transport are an early manifestation in the pathological progression of glaucoma. Previous studies suggest that anterograde and retrograde transport are differentially challenged and pre-degenerative in glaucoma, yet, both forms of transport have never been assessed within the same animal. We used a modified surgical procedure to assess retrograde transport while preserving the structure of the superior colliculus (SC) for both anterograde transport and immunohistochemical analysis in the same optic projection. Our findings demonstrate a 3-fold greater reduction in anterograde transport compared to retrograde transport in 9-10 month old animals. Retrograde transport remained largely intact until 13 months of age, where a reduction similar to anterograde transport was observed. Additionally, immunohistochemical staining revealed that retinal ganglion cell (RGC) axons remained intact until 13 months despite these early transport deficits. Together these data support that the RGC axonal projection remains at least semi-functional and structurally intact after anterograde transport loss in glaucoma. This pre-degenerative loss of transport may provide a target for innovative treatments aimed at restoring function in glaucomatous axons that still remain largely intact.
Samuel Crish, PhD (Advisor)
Christine Crish, PhD (Committee Member)
Denise Inman, PhD (Committee Member)
64 p.
Medicine; Neurobiology; Neurosciences
glaucoma, axonal transport, neurodegeneration, superior colliculus, retina

Recommended Citations

Citations

  • Smith, M. A. (2014). Differential Loss of Bidirectional Axonal Transport with Structural Persistence Within The Same Optic Projection of the DBA/2J Glaucomatous Mouse [Master's thesis, Northeast Ohio Medical University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ne2mh1401723180

    APA Style (7th edition)

  • Smith, Matthew. Differential Loss of Bidirectional Axonal Transport with Structural Persistence Within The Same Optic Projection of the DBA/2J Glaucomatous Mouse. 2014. Northeast Ohio Medical University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ne2mh1401723180.

    MLA Style (8th edition)

  • Smith, Matthew. "Differential Loss of Bidirectional Axonal Transport with Structural Persistence Within The Same Optic Projection of the DBA/2J Glaucomatous Mouse." Master's thesis, Northeast Ohio Medical University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=ne2mh1401723180

    Chicago Manual of Style (17th edition)

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© 2014, all rights reserved.
This open access ETD is published by NEOMED Integrated Pharmaceutical Medicine and OhioLINK.