Produce from gardens can be contaminated by harmful pathogens and cause foodborne illnesses. However, food safety behaviors related to gardening and produce handling have not been studied extensively. One survey focusing on gardeners and produce handlers’ behaviors was administrated. Results indicated that many gardeners tended to practice unsafe gardening behaviors to prevent cross contamination of manure, water, produce, and gardening tools. Although produce handlers tended to possess safer behaviors to handle fresh produce in the kitchens, they still needed to be informed to refrigerate produce properly. Moreover, participants’ gardening and produce handling behaviors, and sources of food safety information about gardening varied in different demographic groups. Through this study, food safety educators can have more understanding of gardeners’ and produce handlers’ food safety behaviors, and design suitable food safety programs or methods in order to effectively deliver food safety knowledge related to produce to specific audiences.