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Solvent Extraction of Lipids from Microalgae

Anthony, Renil J.
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1280854965

Abstract Details

2010, Master of Science (MS), Ohio University, Mechanical Engineering (Engineering and Technology).
Algae are a favorable feedstock for biodiesel due to their high oil content and fast growth rate. The average oil content has been found to be 20-50% of dry weight. Solvent lipid extraction efficiency from algae is strain specific and with the focus on neutral lipids, four non-polar solvents were tested on Oocystis sp. Methylene chloride, hexane, diethyl ether and cyclohexane were chosen, and the extractions performed in a soxhlet apparatus. The lipid extracts were derivatized and analyzed using a combination of Gas Chromatography-Mass Spectrometry. All four solvent extracts showed the same composition of hexadecanoic acid methyl ester (C16:0), linoleic acid methyl ester(C18:2) and linolenic acid methyl ester (C18:3) with the same % wt composition. Among the four, methylene chloride proved to be the most efficient solvent for Oocystis sp. with a total neutral lipid recovery of 0.25% of dry weight followed by diethyl ether,cyclohexane and hexane. The results question the use of the algal strain Oocystis sp. for biofuels. The lipid productivity in Oocystis sp. would need to be verified and a more efficient solvent and/or extraction technique identified, or an algal strain having higher lipid productivity should be employed for biofuel feedstock.
Benjamin Stuart, J (Advisor)
David Bayless, J (Committee Member)
Guy Riefler, R (Committee Member)
Morgan Vis (Committee Member)
101 p.
Botany; Energy; Engineering; Environmental Science; Mechanical Engineering
Algae; Biodiesel; Oocystis; Solvent Extraction; Soxhlet; Microalgae

Recommended Citations

Citations

  • Anthony, R. J. (2010). Solvent Extraction of Lipids from Microalgae [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1280854965

    APA Style (7th edition)

  • Anthony, Renil. Solvent Extraction of Lipids from Microalgae. 2010. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1280854965.

    MLA Style (8th edition)

  • Anthony, Renil. "Solvent Extraction of Lipids from Microalgae." Master's thesis, Ohio University, 2010. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1280854965

    Chicago Manual of Style (17th edition)

ohiou1280854965
2,168
© 2010, all rights reserved.
This open access ETD is published by Ohio University and OhioLINK.