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Using RNA-seq Technology to Explore the Impact of Growth Hormone on Angiogenesis and Other Cellular Pathways in Subcutaneous and Epididymal Adipose Tissue from Wild Type and Bovine Growth Hormone Transgenic Mice

Duran Ortiz, Silvana
http://orcid.org/0000-0001-8870-4077
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1406818100

Abstract Details

2014, Master of Science (MS), Ohio University, Food and Nutrition Sciences (Health Sciences and Professions).
Several studies suggest that inappropriate adipose tissue (AT) vascularization and angiogenesis may contribute to the negative effects of obesity by altering several AT characteristics, such as immune cell infiltration and fibrosis. Growth hormone (GH) has an impact on AT plasticity. That is, disruption of GH action increases AT mass while increased GH action, such as in bovine GH (bGH) mice, decreases AT mass in a depot-specific manner. GH has been implicated in angiogenesis in several tissues; however, to date, no one has investigated the effect of GH on angiogenesis in AT. For this thesis, RNA-seq technology was used to compare the expression levels of angiogenic factors in inguinal (subcutaneous) and epididymal (intra-abdominal) AT depots of male bGH mice to wild type (WT) controls. Because RNA-seq technology also allows one to organize a large amount of data into biologically meaningful groups, this work also classified the significantly altered genes into the predominant cellular processes, networks and pathways that are altered in these animals. The results showed that the inguinal depot of bGH mice have a significant down-regulation of several angiogenic factors including leptin, vascular endothelial growth factor A, vascular endothelial growth factor B and metalloproteinase inhibitor 4 when compared to WT mice. Regarding the more global RNA-seq analysis, the inguinal AT depot of bGH mice had many more significantly altered genes, suggesting a greater impact in this depot. The biological process and pathway analysis of the epididymal AT depot suggested that GH may down-regulate processes related with basic metabolism and organelle organization and biogenesis. In the inguinal AT, the up-regulated genes were related with immune cell activation, especially genes related with T cells. Overall, it was concluded that GH may have a negative impact on the angiogenesis status of the inguinal AT depot of bGH mice, an effect that is depot-dependent. Moreover, GH may decrease the expression levels of genes related with adipocyte differentiation, and increase the expression level of genes associated with T cell activation and regulation. The pathway and process analyses need additional experimental follow-up but provide additional avenues for exploration.
Darlene E. Berryman, Ph.D (Advisor)
John J. Kopchick, Ph.D (Committee Member)
Cheryl A. Howe, Ph.D (Committee Member)
168 p.
Biomedical Research
Adipose tissue; Growth hormone; Angiogenesis; bGH mice; RNA-seq; Pathways

Recommended Citations

Citations

  • Duran Ortiz, S. (2014). Using RNA-seq Technology to Explore the Impact of Growth Hormone on Angiogenesis and Other Cellular Pathways in Subcutaneous and Epididymal Adipose Tissue from Wild Type and Bovine Growth Hormone Transgenic Mice [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1406818100

    APA Style (7th edition)

  • Duran Ortiz, Silvana. Using RNA-seq Technology to Explore the Impact of Growth Hormone on Angiogenesis and Other Cellular Pathways in Subcutaneous and Epididymal Adipose Tissue from Wild Type and Bovine Growth Hormone Transgenic Mice. 2014. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1406818100.

    MLA Style (8th edition)

  • Duran Ortiz, Silvana. "Using RNA-seq Technology to Explore the Impact of Growth Hormone on Angiogenesis and Other Cellular Pathways in Subcutaneous and Epididymal Adipose Tissue from Wild Type and Bovine Growth Hormone Transgenic Mice." Master's thesis, Ohio University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1406818100

    Chicago Manual of Style (17th edition)

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This open access ETD is published by Ohio University and OhioLINK.