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RNA Expression of Receptors for Growth Hormone, Insulin-like Growth Factor 1, and Insulin in Mouse Whole Adipose Tissue, Stromal Vascular Fraction, and Adipocytes

Lesende , Vivian A.
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1440105877

Abstract Details

2015, Master of Science (MS), Ohio University, Biological Sciences (Arts and Sciences).
Increasing rates of obesity and associated complications worldwide have increased research interest in the complex interplay of hormones, metabolism, and adiposity. There is much evidence indicating that hormones such as growth hormone (GH), insulin-like growth factor 1 (IGF-1), and insulin affect white adipose tissue (WAT) in a depot-specific manner. This suggests that hormone-induced intracellular signaling is more active in some WAT depots compared to others, as evidenced by hormone receptor expression levels. To add another layer of complexity, WAT is made up of many cell types with distinct functions, including adipocytes and cells of the stromal vascular fraction (SVF), which can differentially express these receptors. The purpose of this thesis is to further develop our understanding of how GH-, IGF-1-, and insulin-induced signaling affect adiposity in a WAT depot-specific manner. The original aims of this thesis were to: 1) use quantitative polymerase chain reaction (qPCR) to compare the expression of the receptors of the above hormones (GH, IGF-1, and insulin) in the SVF and adipocyte portions of the most commonly studied WAT depots in wildtype (WT) mice (subcutaneous, epididymal, retroperitoneal, and mesenteric), and 2) compare the expression of these receptors in the WAT depots of growth hormone antagonist (GHA) and WT mice. Unfortunately, due to low adipocyte RNA concentrations and purity, the first aim was adjusted to examine receptor RNA expression in whole WAT (as opposed to isolated adipocytes) and SVF. No significant differences in receptor expression were observed in the first aim, but a trend toward greater insulin receptor (IR) RNA expression in the SVF of the epididymal depot compared to the SVF of the mesenteric depot was seen. The main significant finding of the second aim was that growth hormone receptor (GHR) was more strongly expressed in GHA mice than in WT mice in the mesenteric depot, a result that could be explained by differences in SVF cell populations in GHA mesenteric WAT. Thus, future studies should focus on examining differences in WAT immune cell populations in the two genotypes, as well as optimizing the process of RNA isolation from adipocytes to allow for a more clear comparison of the two portions.
Darlene Berryman, PhD (Advisor)
John Kopchick, PhD (Committee Member)
Shigeru Okada, PhD (Committee Member)
Karen Coschigano, PhD (Committee Member)
99 p.
Biology; Molecular Biology
adipose tissue; stromal vascular fraction; growth hormone; insulin-like growth factor 1; insulin receptor; mouse models; adipocytes

Recommended Citations

Citations

  • Lesende , V. A. (2015). RNA Expression of Receptors for Growth Hormone, Insulin-like Growth Factor 1, and Insulin in Mouse Whole Adipose Tissue, Stromal Vascular Fraction, and Adipocytes [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1440105877

    APA Style (7th edition)

  • Lesende , Vivian . RNA Expression of Receptors for Growth Hormone, Insulin-like Growth Factor 1, and Insulin in Mouse Whole Adipose Tissue, Stromal Vascular Fraction, and Adipocytes . 2015. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1440105877.

    MLA Style (8th edition)

  • Lesende , Vivian . "RNA Expression of Receptors for Growth Hormone, Insulin-like Growth Factor 1, and Insulin in Mouse Whole Adipose Tissue, Stromal Vascular Fraction, and Adipocytes ." Master's thesis, Ohio University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1440105877

    Chicago Manual of Style (17th edition)

ohiou1440105877
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© 2015, some rights reserved.Creative Commons License RNA Expression of Receptors for Growth Hormone, Insulin-like Growth Factor 1, and Insulin in Mouse Whole Adipose Tissue, Stromal Vascular Fraction, and Adipocytes by Vivian A. Lesende is licensed under a Creative Commons Attribution-NonCommercial-NoDerivs 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by Ohio University and OhioLINK.