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Graphene Oxide-based Novel Supercapacitor Immunosensors for Physiological Biomarkers Detection

Rodriguez-Silva, Allen A.
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1458922749

Abstract Details

2016, Master of Science (MS), Ohio University, Chemical Engineering (Engineering and Technology).
Two novel electrochemical supercapacitor immunosensors capable of detecting low density lipoprotein (LDL) and D-dimer in vitro were developed. More specifically, graphene oxide (GO) was functionalized with hyperbranched polyethylenimine (PEI), merged with chitosan to obtain a nanocomposite polymeric film and finally dried over a Pt electrode for further treatment. Surface bio-functionalization took place by fixing over the surface, anti- apolipoprotein B100 or anti-fibrinogen antibody, for LDL or D-Dimer specificity respectively, using the N-hydroxysuccimide/N-ethyl-N-(3-dymethylaminopropyl) carbodiimide (EDC/NHS) coupling chemistry approach. The immunosensors operated in the concentration ranges of 10 - 120 mg/dL for LDL and 50 - 500 ng/mL for D-dimer where they showed non-linear but consistent and proportional specific capacitance response for different species concentrations. The material properties of the electrodes were evaluated by Fourier transform infrared (FTIR) spectroscopy, Raman spectroscopy (RS), X-Ray diffraction (XRD), thermogravimetric analysis (TGA), scanning electron microscopy (SEM), and cyclic voltammetry (CV). The developed supercapacitor immunosensors demonstrated specificity for their antigens and response times of less than 2 s.
John Staser, Ph.D. (Advisor)
83 p.
Chemical Engineering
graphene oxide; immunosensor; supercapacitor; low density lipoprotein; D-dimer; electrochemical biosensor

Recommended Citations

Citations

  • Rodriguez-Silva, A. A. (2016). Graphene Oxide-based Novel Supercapacitor Immunosensors for Physiological Biomarkers Detection [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1458922749

    APA Style (7th edition)

  • Rodriguez-Silva, Allen. Graphene Oxide-based Novel Supercapacitor Immunosensors for Physiological Biomarkers Detection. 2016. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1458922749.

    MLA Style (8th edition)

  • Rodriguez-Silva, Allen. "Graphene Oxide-based Novel Supercapacitor Immunosensors for Physiological Biomarkers Detection." Master's thesis, Ohio University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1458922749

    Chicago Manual of Style (17th edition)

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© 2016, all rights reserved.
This open access ETD is published by Ohio University and OhioLINK.