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Effects of the Nanoparticle Protein Corona on Nanoparticle-Cell Membrane Interactions

Haghighat Manesh, Mohamad Javad Haghighat
http://orcid.org/0000-0003-2173-3046
http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1597967288027448

Abstract Details

2020, Master of Science (MS), Ohio University, Biomedical Engineering (Engineering and Technology).
The increasing applications of nanoparticles in biomedical research and consumer products has raised concerns regarding their potential toxicity. Although the effects of nanoparticles on human health and environment are still under investigation, previous studies have revealed significant evidence of nanoparticle toxicity to cells. With the cell plasma membrane being the first entity that first interacts with nanoparticles upon possible exposure, numerous studies have focused on nanoparticle interactions with cell plasma membrane. Nanoparticles entering a biological fluid are covered by proteins which adsorb to the particle surface, commonly called the nanoparticle protein corona. Protein corona changes nanoparticle characteristics, thereby altering their biological behavior. A better understanding of how the corona regulates nanoparticle-membrane interactions can help the design of the biomedical applications of nanoparticles. This study seeks to investigate the role of the corona from human serum on nanoparticle-induced cell membrane damage. Plain silica nanoparticles, known for disrupting the lipid bilayer of the cell membrane, were used as nanoparticle models. The effects of pristine nanoparticles are recorded after interactions with model membranes and red blood cells and compared to results from nanoparticles with the protein corona on their surface. The results show that while the applied silica nanoparticles are disruptive to the cell membrane in their pristine form, the damage to the cell membrane is significantly reduced after the formation of the protein corona. Furthermore, the specific proteins adsorbed on the nanoparticle surface play an important role in the ability of the particles to induce membrane damage with the examination of a number of abundant proteins in the corona, it is concluded that one of the main reasons behind these interactions is the surface coverage of the nanoparticles by proteins.
Amir Farnoud (Advisor)
66 p.
Biomedical Engineering
Nanoparticle; Cell membrane damage; Protein corona; Biomedical Engineering

Recommended Citations

Citations

  • Haghighat Manesh, M. J. H. (2020). Effects of the Nanoparticle Protein Corona on Nanoparticle-Cell Membrane Interactions [Master's thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1597967288027448

    APA Style (7th edition)

  • Haghighat Manesh, Mohamad Javad. Effects of the Nanoparticle Protein Corona on Nanoparticle-Cell Membrane Interactions. 2020. Ohio University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1597967288027448.

    MLA Style (8th edition)

  • Haghighat Manesh, Mohamad Javad. "Effects of the Nanoparticle Protein Corona on Nanoparticle-Cell Membrane Interactions." Master's thesis, Ohio University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=ohiou1597967288027448

    Chicago Manual of Style (17th edition)

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© 2020, all rights reserved.
This open access ETD is published by Ohio University and OhioLINK.