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Complex mechanisms of metabolic regulation in nonperfused muscle

Pasniciuc, Silviu Valeriu
http://rave.ohiolink.edu/etdc/view?acc_num=osu1049304386

Abstract Details

2004, Master of Science, Ohio State University, Biophysics.
The measurement of respiration at the whole tissue level has important value for understanding the physiology of whole tissue preparations. However, there has never been a critical evaluation of the limitations of the measurement, nor has a consistent interpretive strategy been available. The decay of oxygen consumption observed over time when measurements are taken in a closed system (first described by Wittenberg et al. in 1970) is investigated and several hypotheses were tested. Hypoxia was rejected as a possible cause for the decay using different experimental approaches. The possible accumulation of reactive oxygen or nitrogen species was tested, but did not prove to be the main causative factor for this phenomenon. Acid- base changes consistently modified the pattern of the decay and were concluded to be one of the main regulatory factors in the process of cell respiration, with possible applications in well-described pathophysiologic models, like muscle hibernation. The measurement of respiration at the whole tissue level has important value for understanding the physiology of whole tissue preparations. However, there has never been a critical evaluation of the limitations of the measurement, nor has a consistent interpretive strategy been available. The decay of oxygen consumption observed over time when measurements are taken in a closed system (first described by Wittenberg et al. in 1970) is investigated and several hypotheses were tested. Hypoxia was rejected as a possible cause for the decay using different experimental approaches. The possible accumulation of reactive oxygen or nitrogen species was tested, but did not prove to be the main causative factor for this phenomenon. Acid- base changes consistently modified the pattern of the decay and were concluded to be one of the main regulatory factors in the process of cell respiration, with possible applications in well-described pathophysiologic models, like muscle hibernation.
Thomas Clanton (Advisor)
119 p.
oxygen consumption; skeletal muscle; nitric oxide; acid-base changes; muscle hibernation

Recommended Citations

Citations

  • Pasniciuc, S. V. (2004). Complex mechanisms of metabolic regulation in nonperfused muscle [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1049304386

    APA Style (7th edition)

  • Pasniciuc, Silviu. Complex mechanisms of metabolic regulation in nonperfused muscle. 2004. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1049304386.

    MLA Style (8th edition)

  • Pasniciuc, Silviu. "Complex mechanisms of metabolic regulation in nonperfused muscle." Master's thesis, Ohio State University, 2004. http://rave.ohiolink.edu/etdc/view?acc_num=osu1049304386

    Chicago Manual of Style (17th edition)

osu1049304386
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© 2004, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.