Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


osu1117548268.pdf (37.55 MB)

ETD Abstract Container

Abstract Header

Biochemical and structural characterization of novel metalloprotein sensors and carboxypeptidases

Isaza, Clara Eugenia
http://rave.ohiolink.edu/etdc/view?acc_num=osu1117548268

Abstract Details

2005, Doctor of Philosophy, Ohio State University, Biophysics.
The focus of the research that I carried out during my Ph.D. was the characterization of three metalloproteins. The characterization was based on the information obtained from their structures and biochemical data obtained from in-vitro and in-vivo experiments. The structures of the three proteins studied were obtained by x?ray crystallography. The first protein studied was the hemerythrin-like domain from the Desulfovibrio vulgaris chemoreceptor H. To be able to propose a role and the mechanism by which this domain works, we solved the structures of the protein in the three physiological relevant states. The proposed mechanism is based on the observed differences between the three states. The second protein studied was the Bacillus subtilis M32 carboxypeptidase, BsuCP. The activity of this enzyme was tested with different substrates to learn about its specificity and the length of substrates on which it acts. The structure of the protein was solved to understand the properties that influence its activity. The third aim of this Ph.D. work was the study of another M32 carboxypeptidase, LmaCP, which is found in Leishmania major. The substrate specificity for LmaCP was studied by enzymatic assays. To propose a physiological role for this enzyme, its expression profile in the three main life stages of the L. major protozoa was examined. We were able to used the previously solved crystallographic structure of LmaCP (solved previously by this laboratory (Zhong et al, unpublished results)) to compare it with the BsuCP structure to explain the activity differences found between these two members of the M32 family of carboxypeptidases.
Michael Chan (Advisor)
108 p.
Proteins crystal structure; Oxygen sensor; M32 family; Carboxypeptidases

Recommended Citations

Citations

  • Isaza, C. E. (2005). Biochemical and structural characterization of novel metalloprotein sensors and carboxypeptidases [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1117548268

    APA Style (7th edition)

  • Isaza, Clara. Biochemical and structural characterization of novel metalloprotein sensors and carboxypeptidases. 2005. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1117548268.

    MLA Style (8th edition)

  • Isaza, Clara. "Biochemical and structural characterization of novel metalloprotein sensors and carboxypeptidases." Doctoral dissertation, Ohio State University, 2005. http://rave.ohiolink.edu/etdc/view?acc_num=osu1117548268

    Chicago Manual of Style (17th edition)

osu1117548268
456
© 2005, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.