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Novel tissue scaffolds comprising nano- and micro-structures

Ng, Robin
http://rave.ohiolink.edu/etdc/view?acc_num=osu1196260817

Abstract Details

2007, Doctor of Philosophy, Ohio State University, Chemical Engineering.
Extensive research has been conducted in understanding the surface-biotic interactions. Although surface chemistry has long been known to regulate cell growth, development, mobility and differentiation, it is not until recently that the effects of surface topography on cellular behaviors were found. The observed effects of the nanofibrous surfaces on cell culture are very minimal, however, due the short culture period resulted from the insufficient area to sustain cell growth. Three dimensional (3-D) culture of animal cells, at the other end, is another emerging research area in the field of tissue engineering. Although the 3-D culture takes advantages of the abundant surface area and microenvironment provided by the scaffold, the lack of nanostructured surfaces in the 3-D scaffold limits its application. The overall objective of this study was to design a novel scaffold comprising of both nano- and micro-structures for the application in tissue engineering and cell culture. Polyethylene terephthalate (PET) scaffold, with a porosity of ~ 45 micron and fiber diameter of ~ 25 micron, was used as a base scaffold materials. Treatments and modifications of this PET scaffold (wigh high interconnectivity) to yield nano-structures were carried out. Treatments were successful in providing nano-roughness to every single individual fibers of the scaffold without affecting the physical properties of the scaffolds (porosity and pore size). Results obtained from the study concluded the effects of nano-structured surface on cell cultures (human and mouse cells). Cells expressed different phenotypic behaviors when grown on nanofibrous surfaces compared to those on flat surfaces. The fabricated novel scaffolds were also shown to demonstrate both nano and micro features of the scaffolds, taking the advantages of both the nano-structured and three-dimensional microenvironment. Further applications of these novel scaffolds were extended to the culture of murine embryonic stem (ES) cells. Besides, the long term culture of ES cells was carried out for approximately 30 days. Continuous perfusion culture of the ES cell was performed at a controlled dilution rate in order to maintain the substrate concentration for the cells. This study demonstrated the potential of applying nanotechnology in bioprocess engineering, contributing to the emerging area of bionanotechnology.
Shang-Tian Yang (Advisor)
232 p.
Engineering, Chemical
Astrocyte; scaffold; nanofiber; polyethylene terephthalate

Recommended Citations

Citations

  • Ng, R. (2007). Novel tissue scaffolds comprising nano- and micro-structures [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1196260817

    APA Style (7th edition)

  • Ng, Robin. Novel tissue scaffolds comprising nano- and micro-structures. 2007. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1196260817.

    MLA Style (8th edition)

  • Ng, Robin. "Novel tissue scaffolds comprising nano- and micro-structures." Doctoral dissertation, Ohio State University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=osu1196260817

    Chicago Manual of Style (17th edition)

osu1196260817
2,492
© 2007, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.