Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


osu1198155366.pdf (356.59 KB)

ETD Abstract Container

Abstract Header

Pharmacological and immunological identification of native α7 nicotinic receptors: evidence for homomeric and heteromeric α7 receptors

El-Hajj, Raed A
http://rave.ohiolink.edu/etdc/view?acc_num=osu1198155366

Abstract Details

2007, Master of Science, Ohio State University, Pharmacy.
Controversy surrounds the expression of α7 nicotinic acetylcholine receptors (nAChRs) in adrenal chromaffin cells. In these studies, α7 nAChRs expressed in bovine adrenal chromaffin cells are investigated. Using radiolabeled ligand binding techniques, [125I]α-bungarotoxin(αBGT) binding reaches equilibrium within 4 hours and is saturable with a Kd value of 4.2 nM. Using homologous competition experiments, the Ki for binding of αBGT was 1.9 nM. These data are consistent with the expression of homomeric α7 nAChRs. Methyllycaconatine (MLA), which binds α7 nAChRs with high afinity, inhibits [125I]αBGT binding in a concentration-dependent manner with a Ki of 30.6 nM; this value is ~10 fold higher than the reported affinity of MLA for α7 nAChRs. We also document the ability of bromoacetylcholine (brACh) to alkylate α7 nAChRs, as has been previously demonstrated for bovine adrenal α3β4 nAChRs. When adrenal nAChRs are immunoprecipated with mAb 319, an antibody which recognizes α7 nAChR protein, and then probed with mAb 319 using Western blot analysis, a single band of ~53 kD is identified. When adrenal nAChRs are immunoprecipated with mAb35, an antibody which recognizes α3 and α5 nAChR proteins, and then probed with mAb319 using Western blot analysis, a single band of ~53 kD is identified. Together, these results support the expression of α7 nAChRs in bovine adrenal chromaffin cells. However, these data suggest that the subunit composition of some of these receptors may include heteromeric α7 nAChRs.
Dennis McKay (Advisor)
40 p.

Recommended Citations

Citations

  • El-Hajj, R. A. (2007). Pharmacological and immunological identification of native α7 nicotinic receptors: evidence for homomeric and heteromeric α7 receptors [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1198155366

    APA Style (7th edition)

  • El-Hajj, Raed. Pharmacological and immunological identification of native α7 nicotinic receptors: evidence for homomeric and heteromeric α7 receptors. 2007. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1198155366.

    MLA Style (8th edition)

  • El-Hajj, Raed. "Pharmacological and immunological identification of native α7 nicotinic receptors: evidence for homomeric and heteromeric α7 receptors." Master's thesis, Ohio State University, 2007. http://rave.ohiolink.edu/etdc/view?acc_num=osu1198155366

    Chicago Manual of Style (17th edition)

osu1198155366
774
© 2007, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.