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Molecular Linkage Between Circadian and Photoperiodic Clocks in the Flesh Fly, Sarcophaga bullata

Han, Bing
http://rave.ohiolink.edu/etdc/view?acc_num=osu1218466287

Abstract Details

2008, Doctor of Philosophy, Ohio State University, Entomology.
A non-diapausing (nd) strain of the flesh fly, Sarcophaga bullata, loses the normal daily adult eclosion rhythm as well as the normal photoperiodic diapause response. The inheritance mode of diapause was investigated by crossing this mutant strain with two other rhythmic diapausing strains having different diapause capacities. The crossing results consistently indicated that diapause incidence is inherited in a simple Mendelian pattern, thus a single gene or a small gene cluster controls the seasonal response of diapause in the nd strain. The essential circadian clock genes, period, timeless, cycle and cryptochrome were originally targeted as candidates of the single malfunctioning gene in the nd strain. As the initial step to study the genetic differences among strains, the full length cDNA sequences of the cryptochrome and the cycle genes in S. bullata were obtained. The cDNA of the cryptochrome gene is 1629 base pairs long, which encodes a protein of 542 amino acids. The cycle gene cDNA is 1517 base pairs long, and its product consists of 410 amino acids. Some functional domains are highly conserved. However, no significant differences at the cDNA level were discovered in the four circadian clock genes between the nd strain and the wild-type flies, suggesting that none of these clock genes is the major cause of circadian rhythm loss in the nd strain. Meanwhile, various mutations on the period gene were discovered using another strain of S. bullata. Among these mutations, perd interferes with light detection when determining time of dawn eclosion, while perm2 shifts the daily eclosion time window significantly earlier. These mutations help detailed investigation of functional motifs on the period gene. More interestingly, deletion and insertion mutations at the C-terminal region, as well as their correlations with diapause capacities, were discovered. This region on the period gene is involved in photoperiodism in S. bullata, suggesting that circadian and photoperiodic clocks are related through a shared molecular component.
David Denlinger (Advisor)
Thomas Wilson (Committee Member)
Glen Needham (Committee Member)
91 p.
Entomology
circadian clock; photoperiodic clock; Sarcophaga bullata

Recommended Citations

Citations

  • Han, B. (2008). Molecular Linkage Between Circadian and Photoperiodic Clocks in the Flesh Fly, Sarcophaga bullata [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1218466287

    APA Style (7th edition)

  • Han, Bing. Molecular Linkage Between Circadian and Photoperiodic Clocks in the Flesh Fly, Sarcophaga bullata. 2008. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1218466287.

    MLA Style (8th edition)

  • Han, Bing. "Molecular Linkage Between Circadian and Photoperiodic Clocks in the Flesh Fly, Sarcophaga bullata." Doctoral dissertation, Ohio State University, 2008. http://rave.ohiolink.edu/etdc/view?acc_num=osu1218466287

    Chicago Manual of Style (17th edition)

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© 2008, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.