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osu1246568225.pdf (257.26 KB)
ETD Abstract Container
Abstract Header
Evaluation of Recombinant
Salmonella
Expressing the Flagellar Protein FliC for Enhanced Immune Responses in Commercial Turkeys
Author Info
Kremer, Courtney J.
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=osu1246568225
Abstract Details
Year and Degree
2009, Master of Science, Ohio State University, Animal Sciences.
Abstract
Salmonella enteritidis
is one of the most common causes of human food-borne illness. This organism is often transmitted to humans from poultry products that may have been contaminated pre- or post-harvest. Previous research indicates that oral vaccination of poultry with live attenuated
Salmonella
can confer a high degree of protection to poultry flocks, thus decreasing the risk of infection in humans. The flagellar protein fliC has shown promise in enhancing an immune response and the ability to protect against challenge of wild-type
S. enteritidis
in poultry. Several novel attenuated strains of
S. enteritidis
were developed that express a fliC epitope on the outer membrane protein lamB using the Red recombinase system in combination with overlapping extension PCR. Poults were orally challenged with 10
6
-10
8
cfu/poult of a wild-type S. enteritidis phage-type 13A (Positive Control), Δ
aro
A/Δ
htr
A SE-M2e (ΔSE M2e), or Δ
aro
A/Δ
htr
A SE-fliC-M2e (ΔSE M2e-fliC) on day-of-hatch and d 21 post-hatch. Liver, spleen, and cecal tonsil were aseptically removed on d 7, 14, 21, 28, 35 and 42 post-hatch to determine colonization and organ invasion. Blood samples were collected on days 7, 14, 21, 28, 35 and 42 post-hatch to determine M2e-specific antibody responses. The ΔSE M2e-fliC candidate vaccine strain demonstrated significantly less organ invasion compared to the ΔSE-M2e strain on d 7 (P <0.0001). However, no marked difference in colonization of the cecal tonsils was noted at d 7. From d 21 through d 42, no significant differences were observed in organ invasion between the vaccine candidate strains when compared to SE13A. The ΔSE M2e-fliC candidate vaccine strain demonstrated a significant decrease in colonization of the cecal tonsils when compared to the ΔSE M2e strain on d 14, 21, and 28 post-immunization (P<0.05). Immunized poults exhibited significantly increased M2e-specific IgG responses, which were further enhanced by the simultaneous expression of fliC on days 14 and 28 (P<0.05). Overall, the ΔSE vaccine candidate strains appear to exhibit reduced organ invasion and colonization while effectively stimulating host immune responses when compared to the wild-type parent strain (SE13A).
Committee
Kimberly Cole, PhD (Advisor)
Billy Hargis, PhD/DVM (Committee Member)
Michael Lilburn, PhD (Committee Member)
Pages
68 p.
Subject Headings
Animals
;
Immunology
Keywords
Salmonella
;
fliC
;
vaccine
Recommended Citations
Refworks
EndNote
RIS
Mendeley
Citations
Kremer, C. J. (2009).
Evaluation of Recombinant
Salmonella
Expressing the Flagellar Protein FliC for Enhanced Immune Responses in Commercial Turkeys
[Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1246568225
APA Style (7th edition)
Kremer, Courtney.
Evaluation of Recombinant
Salmonella
Expressing the Flagellar Protein FliC for Enhanced Immune Responses in Commercial Turkeys.
2009. Ohio State University, Master's thesis.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=osu1246568225.
MLA Style (8th edition)
Kremer, Courtney. "Evaluation of Recombinant
Salmonella
Expressing the Flagellar Protein FliC for Enhanced Immune Responses in Commercial Turkeys." Master's thesis, Ohio State University, 2009. http://rave.ohiolink.edu/etdc/view?acc_num=osu1246568225
Chicago Manual of Style (17th edition)
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Document number:
osu1246568225
Download Count:
622
Copyright Info
© 2009, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.