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Functional Characterization of Magnaporthe oryzae Effectors in the Infective Process of Rice

Burbano-Figueroa, Oscar
http://rave.ohiolink.edu/etdc/view?acc_num=osu1299691367

Abstract Details

2011, Master of Science, Ohio State University, Plant Pathology.
Rice is one of the three most important food crops of the world with an increasing worldwide production during the last decade. One of the major constraints in rice production is rice blast disease caused by the fungus Magnaporthe oryzae. During the last decade, the genomic sequence of rice and this pathogen were completed allowing the computational prediction of genes. The bio-molecular function of these predicted genes are largely unknown. M. oryzae genes MGG00194 and MGG03356 were identified as putative effectors of host defense using a protoplast transient expression system. Effectors are predicted to be secreted in the Blast Interfatial Complex in a specific spatiotemporal pattern, secreted during the development of the invasive hyphae or at least during the late stage of appresorium formation and penetration prior to interfacing with the plant cell. Consequently, the aim of this work was to determine how the putative effectors MGG_00194 and MGG_03356 are involved in the pathogenic process using in-planta secretion and functional analysis. M. oryzae strains that were transformed to constitutively express these genes were obtained and the effect of this condition on vegetative growth and pathogenicity was evaluated. Our results suggest that overexpression of MGG_03356 allows the pathogen to evade the host hypersensitive response possibly via degradation of pectin in the cell wall and consequently decreasing the mechanical sensing of the pathogen. Fluorescent expression patterns of these genes using GFP-tagged proteins in transformant strains were obtained in specific pathogen race-cultivar/species interactions. Even when two different strategies for homologous recombination were used, no deletion mutants were obtained.
Thomas Mitchell, PhD (Advisor)
Anne Dorrance, PhD (Committee Member)
Guo-Liang Wang, PhD (Committee Member)
108 p.
Molecular Biology; Plant Pathology
Magnaporthe oryzae; rice blast; effectors; pectin-lyase

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Citations

  • Burbano-Figueroa, O. (2011). Functional Characterization of Magnaporthe oryzae Effectors in the Infective Process of Rice [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1299691367

    APA Style (7th edition)

  • Burbano-Figueroa, Oscar. Functional Characterization of Magnaporthe oryzae Effectors in the Infective Process of Rice. 2011. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1299691367.

    MLA Style (8th edition)

  • Burbano-Figueroa, Oscar. "Functional Characterization of Magnaporthe oryzae Effectors in the Infective Process of Rice." Master's thesis, Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1299691367

    Chicago Manual of Style (17th edition)

osu1299691367
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© 2011, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.