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Endotoxin Tolerance in Lactating Dairy Cows

Gott, Paige Nicole
http://rave.ohiolink.edu/etdc/view?acc_num=osu1322613488

Abstract Details

2011, Master of Science, Ohio State University, Animal Sciences.
The development of systemic and local endotoxin tolerance (ET) in mid-lactation cows that experienced chronic subacute ruminal acidosis (SARA) or acute SARA and subsequent endotoxin mastitis was investigated. Twenty-four Holstein cows, both primiparous and multiparous, were assigned to eight blocks of three cows grouped by milk production, parity, and days in milk. Cows within blocks were randomly assigned to one of three treatments: 1) Control (Con), 2) High grain (Hi; formulated to reduce milk fat), and 3) Acidosis (designed to reduce milk fat for short periods and potentially induce clinical signs of rumen upset during these periods). Con and Hi treatment animals were fed their respective diets throughout the trial. The Acidosis cows were primarily fed the Con diet, but the Acidosis diet was fed for two two-day periods one week apart. Both Hi and Acidosis diet cows experienced SARA as measured by milk fatty acid profile alterations. Hi cows experienced reduced milk fat percentage, increased trans-10-octadecenoic acid, trans-10, cis-12 conjugated linoleic acid, and increased total trans fatty acids. Acidosis cows had increased trans-10-octadecenoic acid and increased total trans fatty acid content during acidosis bouts. On d 20, all cows were challenged via intramammary infusion with 10 µg of lipopolysaccharide (Escherichia coli 0111:B4) in one mammary quarter 3 h after morning milking. No systemic indicators of ET were observed among treatments. Evidence of an ET response at the local level of the mammary gland was observed. Hi diet animals had reduced milk amyloid A concentration at 12 and 24 h post-LPS in comparison with Con diet cows. Acidosis cows also had lower milk amyloid A concentrations than Con diet cows at 12 h. In addition, Hi diet cows had lower peak somatic cell count at 12 h when compared with Con diet cows. Our data suggest cows which experience varying degrees of SARA and subsequent experimental endotoxin mastitis experience endotoxin tolerance at the level of the mammary gland, but not a systemic reduction in inflammatory mediators.
Joseph S. Hogan, PhD (Advisor)
William P. Weiss, PhD (Committee Member)
Kristy M. Daniels, PhD (Committee Member)
Animal Diseases; Animal Sciences
endotoxin tolerance; mastitis; subacute ruminal acidosis; dairy

Recommended Citations

Citations

  • Gott, P. N. (2011). Endotoxin Tolerance in Lactating Dairy Cows [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1322613488

    APA Style (7th edition)

  • Gott, Paige. Endotoxin Tolerance in Lactating Dairy Cows. 2011. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1322613488.

    MLA Style (8th edition)

  • Gott, Paige. "Endotoxin Tolerance in Lactating Dairy Cows." Master's thesis, Ohio State University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=osu1322613488

    Chicago Manual of Style (17th edition)

osu1322613488
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© 2011, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.