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Fine Mapping and Characterization of fw3.2, One of the Major QTL Controlling Fruit Size in Tomato

Zhang, Na
http://rave.ohiolink.edu/etdc/view?acc_num=osu1332519563

Abstract Details

2012, Doctor of Philosophy, Ohio State University, Horticulture and Crop Science.
Tomato (Solanum lycopersicum) is an important crop in the Solanaceae family. Fruit size is one of the most important agricultural traits controlled by quantitative trait loci (QTL). Therefore, identification of the underlying genes of the major fruit-size loci may benefit the breeding industry, as well as help us better understand the molecular mechanism underlying fruit development. In this study, one of the major fruit-size loci in tomato, fw3.2, was fine mapped by linkage analysis to a 51.4 kb interval corresponding to BAC HBa0051C24 in the tomato genome. The gene action suggested a gain-of-function mutation occurred in cultivar allele producing larger fruit during the domestication. The phenotypic characterization of near isogenic lines (NILs) showed that this locus also controls other traits such as branch number, leaf size and seed size. Yield per plant was similar and the larger fruited lines carried fewer fruit that ripened later than the smaller fruited lines. The changes in fruit weight were not due to an alteration in the sink-source relationship. Expression level analysis of the 7 candidate genes in the NILs did not identify which gene may underlie fw3.2 and numerous SNPs and Indels were found between the parents of the population. Based on function of the putative orthologs, one candidate gene is proposed to be FW3.2. Association mapping around this candidate gene yielded one quantitative trait nucleotide (QTN) in the promoter of the gene. Further genetic analysis of this QTN supported the finding that this SNP is the causative mutation at the fw3.2 locus.
Esther Van der Knaap (Advisor)
Eric Stockinger (Committee Member)
David Francis (Committee Member)
Guo-liang Wang (Committee Member)
152 p.
Genetics; Plant Biology
QTL; fine mapping; fruit size; organ size; tomato

Recommended Citations

Citations

  • Zhang, N. (2012). Fine Mapping and Characterization of fw3.2, One of the Major QTL Controlling Fruit Size in Tomato [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1332519563

    APA Style (7th edition)

  • Zhang, Na. Fine Mapping and Characterization of fw3.2, One of the Major QTL Controlling Fruit Size in Tomato. 2012. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1332519563.

    MLA Style (8th edition)

  • Zhang, Na. "Fine Mapping and Characterization of fw3.2, One of the Major QTL Controlling Fruit Size in Tomato." Doctoral dissertation, Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1332519563

    Chicago Manual of Style (17th edition)

osu1332519563
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© 2012, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.