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Molecular and genetic analyses of freezing tolerance in the Triticeae cereals

Dhillon, Taniya
http://rave.ohiolink.edu/etdc/view?acc_num=osu1354635212

Abstract Details

2012, Doctor of Philosophy, Ohio State University, Horticulture and Crop Science.
Freezing tolerance and winter hardiness are complex traits. Over the course of the last 100 years different genetic tools have been employed with cereals of the Triticeae to gain insight into the complex nature of these traits. Emerging from many independent studies are findings that large affects are contributed by two regions on group five chromosome homoeologs, now identified as Frost Resistance-1 (FR-1) and FR-2. The FR-2 locus encompasses a cluster of genes encoding at least 11 different C-Repeat Binding Factor (CBF) proteins while FR-1 exerts negative control over expression of the CBF genes at FR-2. Recently we determined the physical structure of collinear segments of the FR-H2 region from four barley cultivars. Two of these were from freezing-tolerant winter-hardy genotypes and two of these were from less freezing-tolerant nonwinter-hardy genotypes. A key finding was that the genomic region encompassing subsets of the genes at FR-H2 is amplified to multiple copies occurring in tandem in the freezing-tolerant winter-hardy genotypes whereas the collinear genomic regions in spring genotypes occur in single copy. Other data suggest increased copy numbers are associated with increased transcript levels of the other CBF genes at FR-H2. Our data also indicate CBF copy number variations in wheat. Taken together, these data suggest that CBF copy number variation is a recurring theme in the Triticeae, and that total CBF copy number rather than a particular CBF may be the molecular basis underlying FR-2.
Eric J. Stockinger (Advisor)
Esther van der Knaap (Committee Member)
David M. Mackey (Committee Member)
Guo-Liang Wang (Committee Member)
291 p.
Molecular Biology; Plant Biology
Freezing tolerance; CBF; barley; wheat; Triticeae

Recommended Citations

Citations

  • Dhillon, T. (2012). Molecular and genetic analyses of freezing tolerance in the Triticeae cereals [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1354635212

    APA Style (7th edition)

  • Dhillon, Taniya. Molecular and genetic analyses of freezing tolerance in the Triticeae cereals. 2012. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1354635212.

    MLA Style (8th edition)

  • Dhillon, Taniya. "Molecular and genetic analyses of freezing tolerance in the Triticeae cereals." Doctoral dissertation, Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1354635212

    Chicago Manual of Style (17th edition)

osu1354635212
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© 2012, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.