Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


osu1354643898.pdf (14.8 MB)

ETD Abstract Container

Abstract Header

Three-Dimensional Matrices Used to Characterize Cellular Behavior

Stevenson, Mark Daniel
http://rave.ohiolink.edu/etdc/view?acc_num=osu1354643898

Abstract Details

2012, Doctor of Philosophy, Ohio State University, Biomedical Engineering.
Two-dimensional cell culture is inadequate for characterizing cellular behavior because cells are forced into a flattened morphology unnatural compared to their native environment. Three-dimensional cell culture is more appropriate as cells are encapsulated in a matrix that is more representative of natural conditions in the body. In one of our studies, we use a natural extracellular matrix protein, collagen, to test a model that hypothesizes that cells remodel the matrix in their pericellular region and stop compacting when a desired matrix concentration is reached. We use confocal microscopy to confirm matrix compaction in the pericellular regions. Our model confirms that a highly contractile cell type, fibroblasts, exert higher traction forces and exhibit more compaction than a lower contractile cell type, endothelial cells. In another study, we use a self-assembling peptide matrix to independently control stiffness and binding site density. This matrix is different from natural extracellular matrices in that natural matrices exhibit changes in fiber morphology and pore size as stiffness is changed while our self-assembling peptide matrix does not. We use this matrix to investigate microvascular network formation as a function of matrix stiffness. Microvascular networks are measured using confocal microscopy. Our results show that increased matrix stiffness decreases the extent of microvascular network formation. Another study examines the effect of tissue culture on endothelium dependent relaxation of rat aortas using myography. Results show that vessel activity decreases after two days in culture. Results also show that decreased vessel relaxation in response to oxidized low density lipoproteins see with fresh vessels is maintained. In our final study we cultured human and porcine saphenous veins for up to two weeks to study intimal hyperplasia formation. Our results do not show that intimal hyperplasia formed in that time, in conflict with the available literature. Another result from this study is the development of a perivascular wrap that would be capable of serving as a drug delivery system for the prevention of intimal hyperplasia.
Keith Gooch (Advisor)
Rita Alevriadou (Committee Member)
Mark Ruegsegger (Committee Member)
Alan Litsky (Committee Member)
Irena Levitan (Committee Member)
153 p.
Biomedical Engineering
3D cell culture; collage; self-assembling peptides; microvascular networks; intimal hyperplasia; myography

Recommended Citations

Citations

  • Stevenson, M. D. (2012). Three-Dimensional Matrices Used to Characterize Cellular Behavior [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1354643898

    APA Style (7th edition)

  • Stevenson, Mark. Three-Dimensional Matrices Used to Characterize Cellular Behavior. 2012. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1354643898.

    MLA Style (8th edition)

  • Stevenson, Mark. "Three-Dimensional Matrices Used to Characterize Cellular Behavior." Doctoral dissertation, Ohio State University, 2012. http://rave.ohiolink.edu/etdc/view?acc_num=osu1354643898

    Chicago Manual of Style (17th edition)

osu1354643898
647
© 2012, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.