Skip to Main Content
 

Global Search Box

 
 
 

ETD Abstract Container

Abstract Header

Inflammatory and Immune Effects of Intra-Articular Mesenchymal Stem Cells in Horses

Pigott, John H
http://rave.ohiolink.edu/etdc/view?acc_num=osu1365030665

Abstract Details

2013, Master of Science, Ohio State University, Veterinary Clinical Sciences.
Mesenchymal stem cells have demonstrated anti-inflammatory and immunomodulatory capabilities as well as modest efficacy in animal models of joint injury, warranting further study as a potential treatment of equine joint disease. The goal of the study is to investigate the inflammatory and immune response to intra-articular injection of autologous, genetically modified (bone morphogenetic protein 2) autologous, allogeneic, and xenogeneic bone marrow-derived mesenchymal stem cells (MSC) in horses. The study group consisted of 6 five-year-old Thoroughbred mares, free of musculoskeletal disease in the fetlocks. The 4 fetlock joints were injected on day 0 with 15 million MSC from the described MSC groups and were assessed for 28 days for clinical and inflammatory parameters representing synovitis, swelling and pain. Arthroscopic examination and synovial biopsies were taken at day 60 post-injection for histologic and molecular analyses. On day 120, peripheral blood mononuclear cells were isolated from each horse and co-cultured with monolayers of each MSC group for analysis of CD4 positive cells via flow cytometry and cytokines via ELISA. There was no significant difference between autologous and genetically modified autologous MSC for any parameter examined and soluble gene product was detected for at least 2 days. Allogeneic and xenogeneic MSC produced a modest increase in peak of inflammation at 24 hours than either autologous MSC group. Most inflammatory parameters returned to baseline or control values within 7-14 days for all MSC groups. Arthroscopic examination revealed normal synovium with no grossly detrimental effect to the synovium or cartilage. Synovial histology demonstrated significantly increased inflammatory cells, lymphocyte perivascular cuffing and bone morphogenetic protein 2 antibody uptake for MSC injected joints compared to controls. Adaptive immunity was demonstrated upon re-exposure of peripheral blood mononuclear cells to xenogeneic MSC in co-culture but not for allogeneic MSC. Genetically engineered MSC can produce gene product to detectable synovial concentrations and did not result in greater inflammation warranting further investigation into the therapeutic potential of this cell therapy. Intra-articular MSC resulted in a moderate acute inflammatory joint response that was greater for allogeneic and xenogeneic MSC than autologous MSC. Clinical management of this response may minimize this effect. Intra-articular MSC demonstrated a persistent mononuclear infiltrate for at least 60 days, the significance of which is not known. An adaptive immune response was detected for xenogeneic but not for allogeneic MSC suggesting that a second intra-articular injection of xenogeneic MSC would result in a stronger inflammatory response.
Alicia Bertone, DVM, PhD, DACVS (Advisor)
Maxey Wellman, DVM, PhD, DACVP (Committee Member)
Duncan Russell, BVMS (hons), DACVP (Committee Member)
38 p.
Veterinary Services
Mesenchymal stem cell; equine; joint; inflammation

Recommended Citations

Citations

  • Pigott, J. H. (2013). Inflammatory and Immune Effects of Intra-Articular Mesenchymal Stem Cells in Horses [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1365030665

    APA Style (7th edition)

  • Pigott, John. Inflammatory and Immune Effects of Intra-Articular Mesenchymal Stem Cells in Horses. 2013. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1365030665.

    MLA Style (8th edition)

  • Pigott, John. "Inflammatory and Immune Effects of Intra-Articular Mesenchymal Stem Cells in Horses." Master's thesis, Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1365030665

    Chicago Manual of Style (17th edition)

osu1365030665
304
© 2013, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.