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RanLi_MScThesis OSU.pdf (1.5 MB)

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Molecular Characterization of Human Tissue Samples by Raman Spectroscopy with Different Laser Modes and Excitation Wavelengths

Li, Ran
http://rave.ohiolink.edu/etdc/view?acc_num=osu1365419543

Abstract Details

, Master of Science, Ohio State University, Chemistry.
In recent years, Raman spectroscopy has emerged as a potentially useful tool for the detection and pathological diagnosis of cancerous tissues. This technique has the merit of being highly sensitive to the biochemical alterations in tissue compositions. Nevertheless, the use of Raman spectroscopy in cancer detection has been impeded by high background intensity which can mask the Raman signal of biological molecules. In this study, we aimed at investigating the origins of background and reducing it by studying the influence of both excitation wavelengths and optical modes. Different optical modes, single mode and multimode lasers, with the same excitation 785 nm and different excitation wavelengths, 785 nm and 830 nm lasers with the same multimode are compared here. According to this study, autofluorescence and multiple Mie scattering give rise to the high background intensities in the Raman spectra. The reduction in autofluorescence is demonstrated in the comparison between 785 nm multimode (785 MM) and 830 nm multimode (830 MM) lasers. 830 MM demonstrates better autofluorescence reduction ability than 785 MM. However, the Raman signal intensity is subsequently reduced by 830 MM. Therefore, the SNRs are not improved in spectra taken by 830 MM than by 785 MM. The reduction in multiple Mie scattering is demonstrated in the comparison between 785 MM and 785 nm single mode (785 SM). 785 SM demonstrates better Mie scattering reduction ability than 785 MM, with equal Raman signal intensities.
Heather Allen (Advisor)
James Coe (Committee Member)
Chemistry

Recommended Citations

Citations

  • Li, R. (2013). Molecular Characterization of Human Tissue Samples by Raman Spectroscopy with Different Laser Modes and Excitation Wavelengths [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1365419543

    APA Style (7th edition)

  • Li, Ran. Molecular Characterization of Human Tissue Samples by Raman Spectroscopy with Different Laser Modes and Excitation Wavelengths. 2013. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1365419543.

    MLA Style (8th edition)

  • Li, Ran. "Molecular Characterization of Human Tissue Samples by Raman Spectroscopy with Different Laser Modes and Excitation Wavelengths." Master's thesis, Ohio State University, 2013. http://rave.ohiolink.edu/etdc/view?acc_num=osu1365419543

    Chicago Manual of Style (17th edition)

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© 2013, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.