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Proteomic analysis of 1-D Sarcoplasmic Protein Profiles of Pekin Duck Embryos’ Pectoralis Muscle as Influenced by Incubation Temperature

Cheng, Yang
http://rave.ohiolink.edu/etdc/view?acc_num=osu1407854892

Abstract Details

2014, Master of Science, Ohio State University, Animal Sciences.
The objective of this study was to identify sarcoplasmic proteins responsive to incubation temperature in Pekin duck embryos. Previous studies reported that a 1-degree Celsius increase in incubation temperature during the first 10 days can accelerate embryonic development and this study was designed to identify the effects of early incubation temperature on embryonic myogenesis. Pekin duck eggs were incubated at 37.5 degrees Celsius or 38.5 degrees Celsius for the first ten days and subsequently transferred to 37.5 degrees Celsius for the rest of incubation (ED 11-28). The embryonic pectoralis muscle (PM) was collected at ED12, 18, 25 and hatch and sarcoplasmic proteins were subjected to 10% SDS-PAGE. Gels were digitized into TotalLabTM to acquire the mean band percentage (MBP) of bands. The body weight (BW) of embryos and pectoralis muscle weight (PMW) of the Pekin duck embryos were analyzed in SAS 9.3. An acceleration in BW at ED12 in the 38.5 degrees Celsius treatment was observed but not at later ages. MIXED model is performed to determine bands responding significantly to incubation temperature. Three proteins/bands are determined to significantly respond to temperature.
Michael Lilburn (Committee Chair)
Macdonald Wick (Committee Co-Chair)
William Pope (Committee Member)
64 p.
Animal Sciences

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Citations

  • Cheng, Y. (2014). Proteomic analysis of 1-D Sarcoplasmic Protein Profiles of Pekin Duck Embryos’ Pectoralis Muscle as Influenced by Incubation Temperature [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1407854892

    APA Style (7th edition)

  • Cheng, Yang. Proteomic analysis of 1-D Sarcoplasmic Protein Profiles of Pekin Duck Embryos’ Pectoralis Muscle as Influenced by Incubation Temperature. 2014. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1407854892.

    MLA Style (8th edition)

  • Cheng, Yang. "Proteomic analysis of 1-D Sarcoplasmic Protein Profiles of Pekin Duck Embryos’ Pectoralis Muscle as Influenced by Incubation Temperature." Master's thesis, Ohio State University, 2014. http://rave.ohiolink.edu/etdc/view?acc_num=osu1407854892

    Chicago Manual of Style (17th edition)

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This open access ETD is published by The Ohio State University and OhioLINK.