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Dr_Menendez_Dissertation_12022015.pdf (5.4 MB)

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Multimodal Quantitative Imaging in a Canine Model of Osteoarthritis

Menendez, Maria Isabel
http://rave.ohiolink.edu/etdc/view?acc_num=osu1449150355

Abstract Details

2015, Doctor of Philosophy, Ohio State University, Comparative and Veterinary Medicine.
Osteoarthritis (OA) of the knee is a major public health problem that primarily affects the elderly. Almost 10% of the U. S. population suffers from symptomatic knee OA by the age of 60. There are no approved interventions that ameliorate structural progression of this disorder. The increasing importance of imaging in animal models of osteoarthritis for diagnosis, prognostication, and follow-up is of paramount importance and plays a crucial role in increasing our understanding of the etiology of OA and in the development of new therapies. A primary aim of this study was to provide a comprehensive imaging analysis of the whole knee joint serially in a surgically induced in vivo canine model of OA. We elucidated that quantitative magnetic resonance imaging (MRI) markers demonstrated early changes in the cartilage of the knees that underwent anterior cruciate ligament transection (ACLT) relative to the control knee. This study provided evidence that T2 mapping and delayed gadolinium-enhanced MRI of cartilage (dGEMRIC) are imaging markers relevant to the initiation and progression of OA. Conventional radiography knee assessment, the gold standard in OA diagnosis showed OA signs at a later stage of OA, lacking evidence of premature signs of OA. Serial in vivo imaging utilizing 2-deoxy-2-[fluorine-18] fluoro- D-glucose (18F-FDG) and sodium 18 F-fluoride (18F-NaF) Positron Emission Tomography /Computed Tomography (PET/CT) were performed to characterize knee metabolic and remodeling activity. PET was co-registered with MRI to allow us to improve the location of the regions of interest, otherwise unattainable with PET alone. This work demonstrated, providing imaging evidence, that 18F-FDG and 18F-NaF served an important role in detecting early OA metabolic and remodeling changes in the knee prior to the expression of gross changes. These in vivo changes, in addition to ex vivo micro-PET/CT using 18F-NaF and histomorphometry assessment provided a more valuable understanding of OA. Radiography in combination with clinical imaging technologies, such as, MRI, PET and microcomputed tomography (µCT) produced multimodal imaging techniques that allowed to merge molecular, functional, and anatomical data. These technologies provide a more precise and rigorous methods for exploring OA animal models in greater depth. Collectively, these findings can be interpreted as strong evidence that imaging markers play an important role in post-traumatic OA and that these markers, aimed to detect early signs in OA, may be used clinically to diagnose and follow up therapy treatments in OA.
Michael Knopp (Advisor)
Michael Tweedle (Committee Member)
Thomas Rosol (Committee Member)
David Flanigan (Committee Member)
130 p.
Biomedical Research; Medical Imaging
osteoarthritis; knee; dog; MRI; PET; CT

Recommended Citations

Citations

  • Menendez, M. I. (2015). Multimodal Quantitative Imaging in a Canine Model of Osteoarthritis [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1449150355

    APA Style (7th edition)

  • Menendez, Maria. Multimodal Quantitative Imaging in a Canine Model of Osteoarthritis. 2015. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1449150355.

    MLA Style (8th edition)

  • Menendez, Maria. "Multimodal Quantitative Imaging in a Canine Model of Osteoarthritis." Doctoral dissertation, Ohio State University, 2015. http://rave.ohiolink.edu/etdc/view?acc_num=osu1449150355

    Chicago Manual of Style (17th edition)

osu1449150355
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© 2015, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.
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