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Decellularization to Produce Biological Synovial Extracellular Matrix Scaffolds

Reisbig, Nathalie Ann
http://rave.ohiolink.edu/etdc/view?acc_num=osu1461136148

Abstract Details

2016, Master of Science, Ohio State University, Comparative and Veterinary Medicine.
The objective of this study was to evaluate four methods to generate a decellularized synovium scaffold (SynECM) for use as a biologic transplant. Villous synovium was harvested and frozen (-80oC) from the femoropatellar and medial femorotibial joints of four adult normal horses <7 years of age. Fresh-thawed equine stifle synovial tissue was decellularized by four methods: 1) 0.1% peracetic acid (PAA) solution (1XPAA), 2) PAA treatment repeated (2XPAA), 3) 1% Triton X followed by DNAse (Triton), and 4) 2M NaCl followed by DNAse (NaCl). Tissue from each method was evaluated for morphology (histologic, scanning electron microscopy), viability (culture and exclusion staining) and decellularization efficiency (presence of residual cells, DNA content, and DNA fragmentation). All four methods resulted in non-viable synovial extracellular matrix scaffolds. Single PAA treatment retained synovium villous matrix integrity but with excess cell residue containing high cellular DNA content and DNA fragments > 25,000 base pairs (bp). 2XPAA treatment also had retained matrix integrity, but low DNA content with short DNA fragments (< 300 bp). The Triton and the NaCl preparations damaged villous structure leaving little to no discernible synovial villi, no identifiable residual cells and short (<300 bp) DNA fragments. The results indicated that two serial treatments of 0.1% PAA was the best method evaluated for obtaining SynECM with desirable morphology and low DNA content. An additional advantage of 2XPAA preparation is that PAA sterilizes ECMs during the decellularization process.
Alicia Bertone (Advisor)
30 p.
Animal Sciences
Decellularization; Synovium; Extracellular Matrix; Scaffolds

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Citations

  • Reisbig, N. A. (2016). Decellularization to Produce Biological Synovial Extracellular Matrix Scaffolds [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1461136148

    APA Style (7th edition)

  • Reisbig, Nathalie. Decellularization to Produce Biological Synovial Extracellular Matrix Scaffolds. 2016. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1461136148.

    MLA Style (8th edition)

  • Reisbig, Nathalie. "Decellularization to Produce Biological Synovial Extracellular Matrix Scaffolds." Master's thesis, Ohio State University, 2016. http://rave.ohiolink.edu/etdc/view?acc_num=osu1461136148

    Chicago Manual of Style (17th edition)

osu1461136148
411
© 2016, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.