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Dynamic Aperture Imaging with an Adaptive Optics Scanning Laser Ophthalmoscope as an Approach to Studying Light Scatter in the Retina

Mayne, Danielle Marie
http://orcid.org/0000-0001-7880-8155
http://rave.ohiolink.edu/etdc/view?acc_num=osu1492649742297669

Abstract Details

2017, Master of Science, Ohio State University, Vision Science.
Advances in high resolution retinal imaging reveal features in normal and diseased eyes that were not previously observable, due to resolution limits of imaging systems or the optically transparent nature of many cellular structures in the human retina. Novel imaging techniques can help increase our understanding of disease processes in the eye as well as insight into more generalized systemic changes occurring in neuronal structures and vasculature. Although there are advantages of the resolution in a highly confocal imaging system, some retinal features are more easily distinguished using more multiply scattered light. Multiply scattered light can be collected by offsetting a confocal aperture by varying degrees. It is a challenge to find the best imaging modality for the features of interest. Even though a highly confocal system usually offers the best axial resolution for optical sectioning, in this study we looked at the optical sectioning abilities using a relatively large aperture in a confocal and offset orientation in an adaptive optics scanning laser ophthalmoscope imaging system. We found that using the relatively large confocal aperture, that the system was capable of optically sectioning different retinal layers. In the offset orientation, it was also possible for different layers of the retina to be sectioned even though these were imaged with primarily multiply scattered light. This was accomplished using the retinal capillary networks to recognize axial position in the retina. This study also explores the use of a novel imaging technique, dynamic aperture imaging, where the aperture position is varied during image acquisition. Starting in an offset orientation, the aperture is then moved towards the confocal position, to the confocal position, then past it, while the subject fixates on the same target. With this technique, both directly backscattered light as well as multiply scattered light are able to be collected and different retinal features are able to be compared with both modalities. Dynamic aperture imaging introduces a novel approach to quantify and analyze the light scattering properties in different retinal layers. By using an intensity profile comparing the average intensity per pixel with varying offset displacement of the aperture, the scattering of a retinal layer can be quantified. Our results show differences in the scattering of the different retinal layers analyzed.
Dean VanNasdale, OD, PhD (Advisor)
52 p.
Optics
adaptive optics; retinal imaging

Recommended Citations

Citations

  • Mayne, D. M. (2017). Dynamic Aperture Imaging with an Adaptive Optics Scanning Laser Ophthalmoscope as an Approach to Studying Light Scatter in the Retina [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1492649742297669

    APA Style (7th edition)

  • Mayne, Danielle. Dynamic Aperture Imaging with an Adaptive Optics Scanning Laser Ophthalmoscope as an Approach to Studying Light Scatter in the Retina. 2017. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1492649742297669.

    MLA Style (8th edition)

  • Mayne, Danielle. "Dynamic Aperture Imaging with an Adaptive Optics Scanning Laser Ophthalmoscope as an Approach to Studying Light Scatter in the Retina." Master's thesis, Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1492649742297669

    Chicago Manual of Style (17th edition)

osu1492649742297669
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© 2017, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.