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Shroom3 Localization and Apical Constriction during the Development of the Crystalline Lens in Mouse Embryos

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2017, Master of Science, Ohio State University, Vision Science.
Purpose: The purpose of this research was to analyze the localization of the protein Shroom3, and determine its role in regulating lens fiber cell shape during the organization and development of the embryonic lens. It has previously been shown in the lens placode that Shroom3 is a crucial component in the pathway that leads to apical constriction. To determine if this is also the case in the lens fiber cells within the lens bow of developing lenses, the localization of Shroom3 expression was first evaluated, followed by an analysis of the degree of apical constriction. Methods: Control and Shroom3-deficient mouse embryos were dissected, cryosectioned through the developing lens, and imaged. The images were analyzed following histological labeling using the x-gal assay, and immunofluorescent labeling of specific proteins including Shroom3, beta-catenin, non-muscle myosin IIb and F-actin. Results: Shroom3 mRNA expression is localized to the cells within the lens epithelium, and the lens fiber cells in contact with the lens fulcrum, but not in older, more centrally located lens fibers. Antibody staining showed Shroom3 protein localization within the membranes of these same cells. This stands in contrast to the more specific apical localization observed in other epithelial tissues. As the cells were differentiating, they changed from a columnar-shaped cell to a conical-shaped cell. F-actin and non-muscle myosin expression was diminished apically and increased basally in the Shroom3 null mice embryos. Differentiating lens fiber cells at the lens fulcrum apically constricted less in the Shroom3 null mouse embryos than the controls. Conclusion: Shroom3 directs apical constriction of lens cells as they differentiate from lens epithelial cells to lens fibers in part by modulating the distribution of F-actin and non-muscle myosin.
Timothy Plageman (Advisor)
Heather Chandler (Committee Member)
Andrew Hartwick (Committee Member)
60 p.

Recommended Citations

Citations

  • Eckes, M. (2017). Shroom3 Localization and Apical Constriction during the Development of the Crystalline Lens in Mouse Embryos [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1492728223255337

    APA Style (7th edition)

  • Eckes, Melissa. Shroom3 Localization and Apical Constriction during the Development of the Crystalline Lens in Mouse Embryos. 2017. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1492728223255337.

    MLA Style (8th edition)

  • Eckes, Melissa. "Shroom3 Localization and Apical Constriction during the Development of the Crystalline Lens in Mouse Embryos." Master's thesis, Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1492728223255337

    Chicago Manual of Style (17th edition)