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The role of LC and FAS in regulating floral meristem and fruit locule number in tomato

Chu, Yi-Hsuan
http://rave.ohiolink.edu/etdc/view?acc_num=osu1512046877370248

Abstract Details

2017, Doctor of Philosophy, Ohio State University, Horticulture and Crop Science.
In tomato, lc and fas control the variation between the small and bilocular fruits from the wild ancestor (S. pimpinellifolium) and large fruit cultivars (S. lycopersicum var. lycopersicum) with up to ten locules. SlWUS and SlCLV3 are the candidates of lc and fas, respectively. The regulatory balance between these two genes plays a pivotal role in meristem maintenance in Arabidopsis. However, the genetic and molecular mechanisms of SlWUS and SlCLV3 have not been functionally characterized in tomato. Here, we performed a detailed phenotypic analysis of the reproductive organs in tomato near-isogenic lines. The results showed that lc and fas synergistically controlled floral organ and locule number. In addition, results from targeted RNA interference (RNAi) and transgenic complementation of fas clearly demonstrated that SlCLV3 was the gene underlying fas. By using mRNA in situ hybridization and transcriptome profiling, we observed temporal and spatial changes in the expression patterns of these two genes during floral development. Our results indicated that lc was a gain-of-function mutation of SlWUS while fas was a loss-of-function mutation of SlCLV3. We also conducted transcriptome analyses to capture differentially expressed genes (DEGs) responding to single and double mutations of lc and fas across vegetative/reproductive meristems and young flower buds. We adopted a recently developed 3’ Tag RNA-seq method for RNA-seq library preparation and compared its performance with the standard whole mRNA-seq method. Gene Ontology analysis over the DEGs showed enriched functionalities related to meristem/floral development and organ patterning. In addition, co-expression analysis revealed that microtubule motor activity and sterol/brassinosteroids biology might underlie differences between wild and fasciated tomato. In summary, the findings on tomato locule number and meristem control in this dissertation have provided new insights into the mechanisms in tomato fruit development.
Esther van der Knaap (Advisor)
Jyan-Chyun Jang (Advisor)
Anna Dobritsa (Committee Member)
David Mackey (Committee Member)
Leah McHale (Committee Member)
150 p.
Horticulture; Plant Biology
Tomato; locule number; LC; FAS; CLV3; WUS; Near-Isogenic Lines; floral meristem

Recommended Citations

Citations

  • Chu, Y.-H. (2017). The role of LC and FAS in regulating floral meristem and fruit locule number in tomato [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1512046877370248

    APA Style (7th edition)

  • Chu, Yi-Hsuan. The role of LC and FAS in regulating floral meristem and fruit locule number in tomato. 2017. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1512046877370248.

    MLA Style (8th edition)

  • Chu, Yi-Hsuan. "The role of LC and FAS in regulating floral meristem and fruit locule number in tomato." Doctoral dissertation, Ohio State University, 2017. http://rave.ohiolink.edu/etdc/view?acc_num=osu1512046877370248

    Chicago Manual of Style (17th edition)

osu1512046877370248
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© 2017, some rights reserved.Creative Commons License The role of LC and FAS in regulating floral meristem and fruit locule number in tomato by Yi-Hsuan Chu is licensed under a Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. Based on a work at etd.ohiolink.edu.
This open access ETD is published by The Ohio State University and OhioLINK.
Release 3.2.12