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MicroRNA Thesis Final.pdf (6 MB)

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MicroRNA expression in the cerebrospinal fluid of dogs with and without Cervical Spondylomyelopathy

Vansteenkiste, Daniella P
http://orcid.org/0000-0001-6506-6455
http://rave.ohiolink.edu/etdc/view?acc_num=osu1554387582013973

Abstract Details

2019, Master of Science, Ohio State University, Comparative and Veterinary Medicine.
Osseous associated cervical spondylomyelopathy (OA-CSM) is a degenerative condition of the cervical vertebral column that affects mainly giant dog breeds. microRNAs (miRNA) are small RNAs that play important gene-regulatory roles, and recent data suggest that circulating miRNAs present in biological fluids may serve as potential biomarkers of neurodegenerative disease. The miRNA profile of normal canine cerebrospinal fluid (CSF) and OA-CSM has not been previously described; therefore, the overarching goal of this study was to characterize the expression levels of miRNAs present in the CSF of Great Danes and identify differentially expressed miRNAs present in the CSF of dogs clinically affected with OA-CSM. Global CSF miRNA expression levels were evaluated in twelve clinically normal dogs and 12 OA-CSM affected dogs using the NanoString nCounter platform. We identified eight miRNAs showing differential expression in OA-CSM dogs compared to clinically normal dogs. MiR-299-5p and miR-765 were upregulated and miR-494, miR-612, miR-302-d, miR-4531, miR-4455 and miR-6721-5p were downregulated in the OA-CSM affected dogs. qRT-PCR was performed to validate the expression levels of two miRNAs (miR-494 and miR-612). We found that miR-494 expression was increased by 1.5 fold in the OA-CSM affected dogs, and the expression of miR-612 was decreased by 1.2 fold in the OA-CSM affected group (P-value= 0.41 and 0.89 respectively). Data generated from our study represent an initial characterization of the global miRNA profile of canine CSF and suggest that a distinct CSF miRNA expression profile is associated with OA-CSM in dogs.
Ronaldo da Costa (Advisor)
Joelle Fenger (Committee Member)
Laurie Cook (Committee Member)
Sarah Moore (Committee Member)
74 p.
Veterinary Services

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Citations

  • Vansteenkiste, D. P. (2019). MicroRNA expression in the cerebrospinal fluid of dogs with and without Cervical Spondylomyelopathy [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1554387582013973

    APA Style (7th edition)

  • Vansteenkiste, Daniella. MicroRNA expression in the cerebrospinal fluid of dogs with and without Cervical Spondylomyelopathy . 2019. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1554387582013973.

    MLA Style (8th edition)

  • Vansteenkiste, Daniella. "MicroRNA expression in the cerebrospinal fluid of dogs with and without Cervical Spondylomyelopathy ." Master's thesis, Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1554387582013973

    Chicago Manual of Style (17th edition)

osu1554387582013973
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This open access ETD is published by The Ohio State University and OhioLINK.