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Keckler_Thesis_2019.pdf (1.77 MB)

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Direct Assessment of Osmotic Pressure within Intervertebral Disc Tissue via a Needle Micro-Osmometer

Keckler, Jesse
http://rave.ohiolink.edu/etdc/view?acc_num=osu1555412949107298

Abstract Details

2019, Master of Science, Ohio State University, Biomedical Engineering.
Low back pain affects nearly 84% of people at some point in their life, yet the underlying mechanisms are not completely understood. Intervertebral disc disease is commonly associated with low back pain and features a decrease in proteoglycan content in the intervertebral disc (IVD). This decrease in proteoglycan content is directly tied to a drop in osmotic pressure and ion concentration, as the proteoglycans, specifically aggrecan, attract ions in solution and resist the compression of the disc. Additionally, the change in pressure in the disc due to diurnal activity has been proven to affect the behavior of disc cells, although the true change in osmotic pressure and ion concentration is still unknown. This study aimed to measure the diurnal change in osmotic pressure and ion concentration in a healthy disc by first verifying the use of a needle micro-osmometer in nucleus pulposus (NP) tissue plugs and then in intact discs. Flux measurements using the micro-osmometer were taken in tissue plugs equilibrated in a range of PEG solutions to determine osmotic pressure and ion concentration. The process was then repeated in intact IVDs that were mechanically loaded in a diurnal compression cycle. Osmometer flux measurements in NP tissue had a significant relationship with both osmotic pressure and ion concentration, as did intact motion segments. Variation in motion segment measurements were high, restricting use as a predictive tool. The needle micro-osmometer was proven to measure osmotic pressure and ion concentration in both intact IVDs and tissue plugs, although additional testing is needed to reduce error and enhance repeatability in measurements.
Benjamin Walter, PhD (Advisor)
Devina Purmessur, PhD (Committee Member)
Alan Litsky, PhD (Committee Member)
53 p.
Biomechanics; Biomedical Engineering
Osmotic pressure; intervertebral disc; IVD; fixed charge density; FCD; diurnal pressure

Recommended Citations

Citations

  • Keckler, J. (2019). Direct Assessment of Osmotic Pressure within Intervertebral Disc Tissue via a Needle Micro-Osmometer [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1555412949107298

    APA Style (7th edition)

  • Keckler, Jesse. Direct Assessment of Osmotic Pressure within Intervertebral Disc Tissue via a Needle Micro-Osmometer. 2019. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1555412949107298.

    MLA Style (8th edition)

  • Keckler, Jesse. "Direct Assessment of Osmotic Pressure within Intervertebral Disc Tissue via a Needle Micro-Osmometer." Master's thesis, Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1555412949107298

    Chicago Manual of Style (17th edition)

osu1555412949107298
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This open access ETD is published by The Ohio State University and OhioLINK.