Skip to Main Content
Frequently Asked Questions
Submit an ETD
Global Search Box
Need Help?
Keyword Search
Participating Institutions
Advanced Search
School Logo
Files
File List
LiveRecovery save of Dissertation_Thesis_Templatecopy FINAL COMP FULL chptr 1-4Rev60aa.asd.pdf (8.45 MB)
ETD Abstract Container
Abstract Header
Secondary Metabolites from
Garcinia daedalanthera
Pierre Leaves and Their Role in Cancer Chemotherapy and Chemoprevention
Author Info
Forestrania, Roshamur Cahyan
Permalink:
http://rave.ohiolink.edu/etdc/view?acc_num=osu1557191838034824
Abstract Details
Year and Degree
2019, Doctor of Philosophy, Ohio State University, Pharmaceutical Sciences.
Abstract
The genus
Garcinia
(family Cluseaceae) grows in tropical forests of Asia and Africa (Hemshekhar
et al
., 2011).
Garcinia
species have received some interest by industry due to producing potential medicinal and pharmacologically active chemical entities (Hemshekhar
et al
., 2011).
Garcinia daedalanthera
Pierre is a native Indonesian plant species that has not been studied for its chemical constituents or biological activities. Therefore, an opportunity was taken to explore the promising potential of this plant, as well as to conduct phytochemical studies, specifically in the area of cancer research. The aim of this study was to isolate active compounds from the leaves of
G. daedalanthera
. Structural analysis of the isolates was performed using 1D- and 2D-NMR, LC-MS and GC-MS, IR, polarimetry, and UV-visible physical and spectroscopic methods. Cytotoxicity-guided fractionation was carried out to prioritize fractions for isolating compounds of interest. The types of cell lines used in this cytotoxicity study were MDA-MB-231 and MCF-7 human breast, DU-145 and PC-3 prostate, HeLa cervical, and HT-29 colorectal cancer cell lines. ROS activity towards HT-29 cells was also observed to provide cell-based targeted biological activity data of the isolates. In this study, ten secondary metabolites were purified using combinations of column chromatography and HPLC. Eight known compounds, docosanedioic acid (
150
), 2,5-dimethylnonadecane (
151
), lupeol (
152
), stigmasterol (
153
), β-sitosterol (
154
), heptadecanoic acid (
156
), hexanedioic acid, 1,6-bis[(2
R
)-ethylhexyl] ester (
158
), and 1,3-di-
O
-[2’,2’-di-(
p
-phenylene)] (
159
) were identified. Also, two new compounds, (
S
)-2-hydroxy-3-(octanoyloxy)propyl pentacosanoate (
155
) and (
S
)-3-(((
S
)-11-acetoxyoctadecanoyl)oxy)propane-1,2-diyl diacetate (
157
) were structurally elucidated. Compound
152
showed weak cytotoxicity towards HT-29 cells with an IC
50
value of 19.2 µM. The other isolated compounds did not exhibit any cytotoxicity towards the cancer cell lines at 20 µg/mL. In a ROS assay, compounds
153
showed ROS induction at 50 µM by ~1.24 fold in ROS-induced HT-29 cells. This result suggested that this compound potentially have a role as adjuvant therapy in cancer related to ROS activation. Compound
156
was found to possess a similar level of ROS induction activity (~1.48 fold induction at 0.5 µM) to daunomycin (positive control) (~1.25 fold induction at 0.4 µM) in HT-29 cells both pretreated or untreated by ROS inducers. This suggested that
156
may have beneficial chemotherapeutic activity using other types of cancer cell lines or in other diseases states related to ROS activation. Cell protective effects via ROS inhibition towards HT-29 cell lines pretreated by ROS inducers were observed for compounds
150
,
151
,
155
,
157
, and
158
, in a dose-dependent manner. Compound
151
showed the highest ROS inhibition in HT-29 cells (36.12%) followed by compound
157
(10%), at 50 µM. These compounds potentially had a chemopreventive action in cancer management. This research represents the first findings of the investigation of potential cancer chemopreventive and chemotherapeutic properties of
G. daedalanthera
leaves via ROS activity.
Committee
Esperanza J. Carcache de Blanco (Advisor)
A. Douglas Kinghorn (Committee Member)
Karl A. Werbovetz (Committee Member)
Harinantenaina L. Rakotondraibe (Committee Member)
Pages
406 p.
Subject Headings
Pharmacy Sciences
Recommended Citations
Refworks
EndNote
RIS
Mendeley
Citations
Forestrania, R. C. (2019).
Secondary Metabolites from
Garcinia daedalanthera
Pierre Leaves and Their Role in Cancer Chemotherapy and Chemoprevention
[Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1557191838034824
APA Style (7th edition)
Forestrania, Roshamur Cahyan.
Secondary Metabolites from
Garcinia daedalanthera
Pierre Leaves and Their Role in Cancer Chemotherapy and Chemoprevention.
2019. Ohio State University, Doctoral dissertation.
OhioLINK Electronic Theses and Dissertations Center
, http://rave.ohiolink.edu/etdc/view?acc_num=osu1557191838034824.
MLA Style (8th edition)
Forestrania, Roshamur Cahyan. "Secondary Metabolites from
Garcinia daedalanthera
Pierre Leaves and Their Role in Cancer Chemotherapy and Chemoprevention." Doctoral dissertation, Ohio State University, 2019. http://rave.ohiolink.edu/etdc/view?acc_num=osu1557191838034824
Chicago Manual of Style (17th edition)
Abstract Footer
Document number:
osu1557191838034824
Download Count:
1,141
Copyright Info
© 2019, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.