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Full text release has been delayed at the author's request until May 13, 2025

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Control of DNA Origami from Self-Assembly to Higher-Order Assembly

Johnson, Joshua A, Dr.
http://orcid.org/0000-0001-9299-7861
http://rave.ohiolink.edu/etdc/view?acc_num=osu1577996668813983

Abstract Details

2020, Doctor of Philosophy, Ohio State University, Biophysics.
Control, and the degree thereof, is a critical factor to characterize for utilizing any technology. As the field of nanotechnology has progressed, the wonderful potential of technologies on the molecular scale have been held back by the difficulty in controlling the precise arrangement and interactions between molecules to create objects with desired properties and functions. The rise of DNA nanotechnology has provided a path to fully realize the potential of nanotechnology through the programmable self-assembly of DNA strands into complex geometries. Specifically, the more recently developed technology known as DNA origami offers a means to create nanoscale structures or mechanisms with nearly any imaginable geometry and with sub-nanometer precision. This thesis focuses on exploring methods for controlling DNA origami on multiple scales. Initially, this thesis will discuss methods for controlling the self-assembly of DNA origami, which can be used to form multiple nanostructures simultaneously from a complex mixture. The relative strengths of self-assembly reactions help illuminate how such processes can be so efficient in simpler contexts. We show that, not only is it possible to fold multiple nanostructures in a single pot but also that the relative yields of each structure can be tuned. We find that the kinetics of folding for small regions of a structure is a dominant factor in these yields. The intermediate portion of this thesis will discuss methods for control of dynamic DNA origami mechanisms. We develop a highly tunable control scheme combining gold nanoparticles and DNA origami hinge mechanisms which is rapid, robust, and repeatable without the need for material additives. We achieve an advancement over previously demonstrated control schemes by reducing the actuation times to the seconds timescale using temperature jump assays. We can more quickly control the hybridization of tunable latching strands compared to strand displacements methods and more readily reverse actuation compared to methods using other material additives. This control scheme forms a basis for more practical nanomanipulation devices and dynamic composites of organic and inorganic materials. The later portion of this thesis will focus on higher-order assemblies of dynamic DNA origami nanomachines. Just as the design space for each nanostructures is nearly infinite, so too is the design space in piecing together multiple nanostructures. We focus on the polymerization of nanoparticle-hinge composites to create multi-dimensional arrays which are rapidly reconfigurable on micron scales. The various ways in which we explore hinge polymerization opens opportunities to study and take advantage of fundamental nanoparticle interactions as well as scale these assembly to, ultimately, create dynamic materials with emergent properties.
Carlos Castro, Dr. (Advisor)
Jessica Winter, Dr. (Committee Member)
Michael Poirier, Dr. (Committee Member)
Ezekiel Johnston-Halperin, Dr. (Committee Member)
271 p.
Biophysics
DNA origami, DNA nanotechnology, nanotechnology, nanoparticles, higher-order, self-assembly, nanomachines, directed assembly

Recommended Citations

Citations

  • Johnson, J. A. (2020). Control of DNA Origami from Self-Assembly to Higher-Order Assembly [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1577996668813983

    APA Style (7th edition)

  • Johnson, Joshua. Control of DNA Origami from Self-Assembly to Higher-Order Assembly. 2020. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1577996668813983.

    MLA Style (8th edition)

  • Johnson, Joshua. "Control of DNA Origami from Self-Assembly to Higher-Order Assembly." Doctoral dissertation, Ohio State University, 2020. http://rave.ohiolink.edu/etdc/view?acc_num=osu1577996668813983

    Chicago Manual of Style (17th edition)

osu1577996668813983
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This open access ETD is published by The Ohio State University and OhioLINK.