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Antioxidant Capacity of Hydrolyzed Whey Protein in Model Infant Formula Powder

Hroncich, Maggie Michelle
http://orcid.org/0000-0003-2005-1449
http://rave.ohiolink.edu/etdc/view?acc_num=osu1620689307398329

Abstract Details

2021, Master of Science, Ohio State University, Food Science and Technology.
The design of food systems prone to lipid oxidation requires a reliable method to analyze antioxidant raw materials and serve as a viable indicator for finished product stability. Twelve commercially available whey protein hydrolysate (WPH) powders were screened for antioxidant activity utilizing a Total Antioxidant Capacity assay kit (Abcam ab65329). The results, which ranged from 350-900 nmol Troxol equivalent/mg, indirectly correlated to the degree of hydrolysis (DH) of the whey protein. The antioxidant capacity method was challenged for its ability to differentiate between variables of a simple oil-in-water system with different antioxidants or prooxidants. Select hydrolysates were utilized at 10% of total protein to manufacture a model infant formula powder containing unsaturated lipids. The infant formula powders were evaluated for oxidation by a trained panel for descriptive sensory analysis over 12 months of storage. The results from the antioxidant capacity of the WPH did not directly correlate with improved oxidative stability in the model infant formula powder. Nonetheless, the partially hydrolyzed whey (21% DH) did exhibit the potential for improved antioxidant protection in infant formula compared to the control with whey protein concentrate. Further research is necessary into whey peptide composition to explore what characteristics of hydrolyzed whey protein can be leveraged to predict stability outcomes for food systems.
Farnaz Maleky (Advisor)
V M Balasubramaniam (Committee Member)
Dennis Heldman (Committee Member)
65 p.
Food Science
Antioxidant Capacity; Hydrolyzed Whey Protein; Infant Formula; Sensory; oxidation; trolox

Recommended Citations

Citations

  • Hroncich, M. M. (2021). Antioxidant Capacity of Hydrolyzed Whey Protein in Model Infant Formula Powder [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1620689307398329

    APA Style (7th edition)

  • Hroncich, Maggie. Antioxidant Capacity of Hydrolyzed Whey Protein in Model Infant Formula Powder. 2021. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1620689307398329.

    MLA Style (8th edition)

  • Hroncich, Maggie. "Antioxidant Capacity of Hydrolyzed Whey Protein in Model Infant Formula Powder." Master's thesis, Ohio State University, 2021. http://rave.ohiolink.edu/etdc/view?acc_num=osu1620689307398329

    Chicago Manual of Style (17th edition)

osu1620689307398329
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This open access ETD is published by The Ohio State University and OhioLINK.
Release 3.2.12