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Magnetic Characterization of Biological and Synthetic Iron-Oxide Nanoparticles

Walsh, Kevin James
http://orcid.org/0000-0002-0621-7230
http://rave.ohiolink.edu/etdc/view?acc_num=osu1650632635002605

Abstract Details

2022, Doctor of Philosophy, Ohio State University, Biophysics.
Detection of iron in biological tissues holds widespread importance in health and disease. The most common methodology employed for iron detection involves histochemical staining for ferritin(iron) in tissue sections. However as summarized in Chapter 1, histochemical analysis of tissue sections in-vitro is often known to mismatch with magnetism-based estimation of iron in-vivo using techniques such a magnetic resonance imaging (MRI). Development of histomagnetic approaches could thus aid a better assessment of iron in-vivo and could complement histochemical analysis in-vitro. In this work we explore the potential of magnetic force microscopy (MFM), a scanning probe, atomic force microscopy-based technique as a histomagnetic tool to assess iron deposits in biological tissues. Although MFM is widely used for solid-state materials and nanoparticles, limited applications exist for biological samples. In Chapter 2, we examine how chemical fixatives commonly used in histology affect histochemical versus histomagnetic mapping. We show that while the iron distribution diffuses due to the use of fixatives, MFM mapping as well as SQUID magnetometry is not affected. In Chapter 3, we extend MFM analysis to detect iron in physiological and pathological tissues. Towards this goal, we utilized rat spleen and injured spinal cord as well as brain tissue from patients with Alzheimer’s disease. Our MFM studies were complemented with histochemical staining as well as electron microscopy analysis. Our results show that size of iv iron deposits in injured spinal cords were smaller in sized accounting in part towards a smaller MFM signal. In Chapter 4, we discuss the unearthing of topographical cross-talk in MFM analysis of tissue sections. To do so we explore the effect of scan rate, topographical roughness, and probe type. Potential methods for eliminating cross-talk artifacts were addressed such as decreasing scan rate and resin-embedded sections as used for electron microscopy sections for MFM. Resin-embedded samples allowed for localization of ferritin(iron) containing macrophages which was verified by their morphological features. In Chapter 5, we develop multimodal MFM approaches. Towards this goal we have developed an indirect MFM methodology and used it to detect clusters of synthetic iron-oxide nanoparticles. Finally, in Chapter 6 we conclude with the strengths and limitations of MFM for biological samples and potential for further studies.
Gunjan Agarwal (Advisor)
122 p.
Biophysics
magnetic force microscopy, iron, biological

Recommended Citations

Citations

  • Walsh, K. J. (2022). Magnetic Characterization of Biological and Synthetic Iron-Oxide Nanoparticles [Doctoral dissertation, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1650632635002605

    APA Style (7th edition)

  • Walsh, Kevin. Magnetic Characterization of Biological and Synthetic Iron-Oxide Nanoparticles. 2022. Ohio State University, Doctoral dissertation. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1650632635002605.

    MLA Style (8th edition)

  • Walsh, Kevin. "Magnetic Characterization of Biological and Synthetic Iron-Oxide Nanoparticles." Doctoral dissertation, Ohio State University, 2022. http://rave.ohiolink.edu/etdc/view?acc_num=osu1650632635002605

    Chicago Manual of Style (17th edition)

osu1650632635002605
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This open access ETD is published by The Ohio State University and OhioLINK.