Skip to Main Content
 

Global Search Box

 
 
 

ETD Abstract Container

Abstract Header

Impact of E-Cigarettes on Oral Wound Healing

Robles, Nerea
http://rave.ohiolink.edu/etdc/view?acc_num=osu1689202066283915

Abstract Details

2023, Master of Science, Ohio State University, Dentistry.
Introduction: E-cigarettes are small, battery-powered devices that deliver a mixture of nicotine, propylene glycol, glycerol, and flavoring agents as aerosols. These devices are being used by ex-smokers, current cigarette smokers, and previous nonsmokers. Previous studies in our laboratory and others have demonstrated that e-cigarette use is associated with high pro-inflammatory response, hypokeratinization of oral epithelium, and increased cytotoxicity, suggesting that these devices can increase the risk for poor postoperative healing outcomes. Objective: The study was aimed to assess oral wound healing in orally and systemically healthy e-cigarette users. Methods: A parallel-arm, prospective clinical study was conducted. 8 subjects using e-cigarettes and 8 age- and gender-matched non-vapers were recruited. Punch biopsy wounds of 5 mm of diameter were created bilaterally in the palatal mucosa opposite to the 2nd premolar. Pre-wounding palatal mucosal swabs were also collected for metabolomics profiling of the microbiome at all time points. Subjects were followed up at 1, 2, 4, 7, 14 and 21 days post-operatively, and 3 mm punch biopsies of the healing wounds were collected from the right and left palate at 1- and 3- weeks respectively. Biopsies were subjected to immunohistochemistry to quantify the levels of vimentin, keratin and filaggrin. Results: Statistically significant differences in overall healing were observed between control and test groups, between visits, as well as different trends shown by the groups over the visits. Significant differences were observed between groups and between visits in terms of bleeding and swelling, while only significant differences between visits were seen for epithelialization and redness. Pain perception and difficulty to obtain hemostasis were more observed in the test group. Pathway analysis between week 1 and 3 revealed that carbohydrate and lipid metabolism was significantly higher in e-cigarette users while synthesis of secondary metabolites was higher in the control group. Immunohistochemistry revealed significantly lower vimentin, filaggrin and keratin scores over all time points in the e-cigarette users when compared to controls. Conclusion: E-cigarette use represents a significant risk to postoperative oral wound healing, affecting keratinization of epithelium, and altering the metabolic profile of the oral microbiome.
Purnima Kumar (Committee Member)
Binnaz Leblebicioglu (Advisor)
Yi-Chu Wu (Committee Member)
90 p.
Dentistry; Health Sciences
electronic cigarettes; oral wound healing; e-cigarette; microbiome; palate

Recommended Citations

Citations

  • Robles, N. (2023). Impact of E-Cigarettes on Oral Wound Healing [Master's thesis, Ohio State University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=osu1689202066283915

    APA Style (7th edition)

  • Robles, Nerea. Impact of E-Cigarettes on Oral Wound Healing. 2023. Ohio State University, Master's thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=osu1689202066283915.

    MLA Style (8th edition)

  • Robles, Nerea. "Impact of E-Cigarettes on Oral Wound Healing." Master's thesis, Ohio State University, 2023. http://rave.ohiolink.edu/etdc/view?acc_num=osu1689202066283915

    Chicago Manual of Style (17th edition)

osu1689202066283915
249
© 2023, all rights reserved.
This open access ETD is published by The Ohio State University and OhioLINK.