Skip to Main Content
 

Global Search Box

 
 
 
 

Files

File List


ouhonors1307562734.pdf (1.37 MB)

ETD Abstract Container

Abstract Header

THE EFFECT OF GROWTH HORMONE ON THE MACROPHAGE CONTENT OF DIFFERENT ADIPOSE TISSUE DEPOTS

Munn, Rachel D.
http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1307562734

Abstract Details

2011, Bachelor of Science (BS), Ohio University, Biological Sciences.
The prominence of obesity has drastically increased during the last decades and is now approaching epidemic proportions. Obesity is highly associated with deleterious health conditions such as type 2 diabetes and atherosclerosis. The greatly increased amount of adipose tissue present in obese individuals exhibits a chronic, low-grade form of inflammation. This inflammation is hypothesized to be the underlying cause of the aforementioned obesity associated comorbidities. The source of this inflammation is thought to be a specific phenotype of macrophages, M1 macrophages, which infiltrate obese adipose tissue and secrete inflammatory cytokines as well as macrophage chemoattractants. Growth hormone drastically decreases the amount of fat mass by inducing lipolysis and prohibiting lipogenesis. Growth hormone also acts on macrophages; however, current research has yet to define the true effect. The purpose of this study is to elucidate the effect of growth hormone on macrophages within adipose tissue. This study uses three transgenic mouse models with varying levels of growth hormone signaling: bGH (bovine growth hormone), GHA (growth hormone antagonist), and GHR-/- (growth hormone receptor knockout). Two adipose tissue depots were dissected from mice of all three genotypes as well as wild type controls. mRNA expression analysis was performed on whole adipose tissue, via PCR Super Arrays. The expression of several macrophage markers was measured to determine the macrophage content and phenotype within adipose tissue. The results obtained in this study showed no significant effect of growth hormone on the macrophage content of either adipose tissue depot studied. However, a differential content of macrophages between adipose tissue depots was seen, coinciding with previous research demonstrating the functional and metabolic differences between adipose tissue depots.
Darlene Berryman, PhD (Advisor)
Biology
growth hormone; macrophages; adipose tissue

Recommended Citations

Citations

  • Munn, R. D. (2011). THE EFFECT OF GROWTH HORMONE ON THE MACROPHAGE CONTENT OF DIFFERENT ADIPOSE TISSUE DEPOTS [Undergraduate thesis, Ohio University]. OhioLINK Electronic Theses and Dissertations Center. http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1307562734

    APA Style (7th edition)

  • Munn, Rachel. THE EFFECT OF GROWTH HORMONE ON THE MACROPHAGE CONTENT OF DIFFERENT ADIPOSE TISSUE DEPOTS. 2011. Ohio University, Undergraduate thesis. OhioLINK Electronic Theses and Dissertations Center, http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1307562734.

    MLA Style (8th edition)

  • Munn, Rachel. "THE EFFECT OF GROWTH HORMONE ON THE MACROPHAGE CONTENT OF DIFFERENT ADIPOSE TISSUE DEPOTS." Undergraduate thesis, Ohio University, 2011. http://rave.ohiolink.edu/etdc/view?acc_num=ouhonors1307562734

    Chicago Manual of Style (17th edition)

ouhonors1307562734
478
© 2011, all rights reserved.
This open access ETD is published by Ohio University Honors Tutorial College and OhioLINK.